Biomaterial Database

[Spectrophotofluorimetric enzymatic determination of total serum bile acids]. 1980

G Annoni, and G Barbi, and G Benzio

A new enzymatic fluorimetric assay for the determination of total serum bile acids is described. The enzyme 3 alpha-idroxysteroid NAD oxireductase by oxydising the free OH in position 3 alpha of bile acid molecule, reduce NAD previously added. Afterwards the hydrogen of the generated NADH is transferred by a diaphorase to resazurin to yield the fluorophore resorufin proportionally to the amount of bile acids in the sample. The assay is simple, accurate, without bilirubin's influence and useful for a routine use because the analysis time is not different from the more common laboratory tests.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D005260	Female		Females
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328	Adult	A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available.	Adults
D001647	Bile Acids and Salts	Steroid acids and salts. The primary bile acids are derived from cholesterol in the liver and usually conjugated with glycine or taurine. The secondary bile acids are further modified by bacteria in the intestine. They play an important role in the digestion and absorption of fat. They have also been used pharmacologically, especially in the treatment of gallstones.	Bile Acid,Bile Salt,Bile Salts,Bile Acids,Acid, Bile,Acids, Bile,Salt, Bile,Salts, Bile
D013050	Spectrometry, Fluorescence	Measurement of the intensity and quality of fluorescence.	Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D015096	3-Hydroxysteroid Dehydrogenases	Catalyze the oxidation of 3-hydroxysteroids to 3-ketosteroids.	3-beta-Hydroxysteroid Dehydrogenase,3 Hydroxysteroid Dehydrogenases,3 beta Hydroxysteroid Dehydrogenase,Dehydrogenase, 3-beta-Hydroxysteroid,Dehydrogenases, 3 Hydroxysteroid,Dehydrogenases, 3-Hydroxysteroid,Hydroxysteroid Dehydrogenases, 3
D045002	3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)	A 3-hydroxysteroid dehydrogenase which catalyzes the reversible reduction of the active androgen, DIHYDROTESTOSTERONE to 5 ALPHA-ANDROSTANE-3 ALPHA,17 BETA-DIOL. It also has activity towards other 3-alpha-hydroxysteroids and on 9-, 11- and 15- hydroxyprostaglandins. The enzyme is B-specific in reference to the orientation of reduced NAD or NADPH.	3 alpha-Hydroxysteroid Dehydrogenase,3 alpha-Hydroxysteroid Dehydrogenase (B-Specific),3 alpha-Hydroxysteroid Oxidoreductase,3alpha-Hydroxysteroid Dehydrogenase (B-specific),5 alpha-3 alpha-Hydroxysteroid Dehydrogenase,5 alpha-Dihydroprogesterone 3 alpha-Hydroxysteroid Oxidoreductase,5-DP-3 alpha-HSO,NADPH-5 alpha-Dihydroprogesterone 3 alpha-Hydroxysteroid Oxidoreductase,3 alpha Hydroxysteroid Dehydrogenase,3 alpha Hydroxysteroid Oxidoreductase,5 alpha Dihydroprogesterone 3 alpha Hydroxysteroid Oxidoreductase,Dehydrogenase, 3 alpha-Hydroxysteroid,NADPH 5 alpha Dihydroprogesterone 3 alpha Hydroxysteroid Oxidoreductase,Oxidoreductase, 3 alpha-Hydroxysteroid