BIOMAT Database Logo BIOMAT Database Logo

Primary structural studies of an H-2L molecule confirm that it is a unique gene product with homology to H-2K and H-2D antigens. 1980

J E Coligan, and T J Kindt, and R Nairn, and S G Nathenson, and D H Sachs, and T H Hansen

Radiochemical methodology has been used in the isolation and preliminary biochemical characterization of the murine H-2Ld major histocompatibility complex gene product. The radiolabeled molecule was isolated by immunoprecipitation from the glycoprotein fraction of detergent-solubilized H-2d tumor cells. Six major CNBr fragments were isolated from a papain fragment of this molecule; three of the fragments are connected by disulfide bonds. Due to the high degree of homology between major transplantation antigens, it was possible to align the fragments by comparison of their amino acid sequences to that of the H-2Kb gene product. Of the positions available for comparison between H-2Ld and H-2Kb, H-2Dd, and H-2Kd gene products, 61 out of 80 (78%), 45 out of 55 (82%), and 12 out of 15 (80%), respectively, are identical. Differences between the Ld and Kb and Dd molecules are distributed throughout the amino acid sequence. These data indicate that the H-2Ld gene product is a molecular species distinct from, but homologous to, the H-2K and H-2D gene products.

UI MeSH Term Description Entries
D010206 Papain A proteolytic enzyme obtained from Carica papaya. It is also the name used for a purified mixture of papain and CHYMOPAPAIN that is used as a topical enzymatic debriding agent. EC 3.4.22.2. Tromasin
D010446 Peptide Fragments Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques. Peptide Fragment,Fragment, Peptide,Fragments, Peptide
D003488 Cyanogen Bromide Cyanogen bromide (CNBr). A compound used in molecular biology to digest some proteins and as a coupling reagent for phosphoroamidate or pyrophosphate internucleotide bonds in DNA duplexes. Bromide, Cyanogen
D005075 Biological Evolution The process of cumulative change over successive generations through which organisms acquire their distinguishing morphological and physiological characteristics. Evolution, Biological
D005796 Genes A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms. Cistron,Gene,Genetic Materials,Cistrons,Genetic Material,Material, Genetic,Materials, Genetic
D006183 H-2 Antigens The major group of transplantation antigens in the mouse. H2 Antigens,Antigens, H-2,Antigens, H2,H 2 Antigens
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus

Related Publications

J E Coligan, and T J Kindt, and R Nairn, and S G Nathenson, and D H Sachs, and T H Hansen
Structural relation of murine "third locus" (H-2L) major histocompatibility antigens to the products of H-2K and H-2D loci.
November 1980, Journal of immunology (Baltimore, Md. : 1950),
J E Coligan, and T J Kindt, and R Nairn, and S G Nathenson, and D H Sachs, and T H Hansen
Skin graft enhancement studies with antigenic differences arising from the H-2K, H-2D, and H-2L loci.
June 1980, Transplantation,
J E Coligan, and T J Kindt, and R Nairn, and S G Nathenson, and D H Sachs, and T H Hansen
Enhancement and antibody-mediated rejection of mouse skin allografts with anti-H-2K, H-2D, H-2L antibodies.
March 1981, Transplantation proceedings,
J E Coligan, and T J Kindt, and R Nairn, and S G Nathenson, and D H Sachs, and T H Hansen
Serological cross-reaction between H-2D and H-2K region antigens.
March 1973, Transplantation proceedings,
J E Coligan, and T J Kindt, and R Nairn, and S G Nathenson, and D H Sachs, and T H Hansen
Molecular independence of H-2K and H-2D antigens on the cell surface.
March 1973, Transplantation proceedings,
J E Coligan, and T J Kindt, and R Nairn, and S G Nathenson, and D H Sachs, and T H Hansen
Enhanced expression of H-2K and H-2D antigens on reticulocytes infected with Plasmodium yoelii.
April 1983, Nature,
J E Coligan, and T J Kindt, and R Nairn, and S G Nathenson, and D H Sachs, and T H Hansen
Interaction of monoclonal antibodies with MHC class I antigens on mouse spleen cells. II. Levels of expression of H-2K, H-2D, and H-2L in different mouse strains.
January 1985, Journal of immunology (Baltimore, Md. : 1950),
J E Coligan, and T J Kindt, and R Nairn, and S G Nathenson, and D H Sachs, and T H Hansen
H-2K and H-2D antigens are independently regulated in mouse embryo fibroblasts.
January 1985, Experimental and clinical immunogenetics,
J E Coligan, and T J Kindt, and R Nairn, and S G Nathenson, and D H Sachs, and T H Hansen
Qa-1-associated antigens. II. Evidence for functional differentiation from H-2K and H-2D antigens.
September 1979, Journal of immunology (Baltimore, Md. : 1950),
J E Coligan, and T J Kindt, and R Nairn, and S G Nathenson, and D H Sachs, and T H Hansen
Alternative pre-mRNA splicing of Kj eliminates a sequence that is unique to H-2K transcripts.
February 1994, Molecular immunology,
Copied contents to your clipboard!