Biomaterial Database

Purification and characterization of rhodanese from Acinetobacter calcoaceticus. 1980

P A Vandenbergh, and R S Berk

Rhodanese (thiosulfate : cyanide sulfur transferase, EC 2,8,1,1) was found to be contitutively present as an intracellular enzyme in Acinetobacter calcoaceticus. The soluble enzyme was purified 40.9-fold by a procedure which included ultracentrifugation, ethanol precipitation, CM-Sephadex batchwise separation, QAE-50 ion exchange chromatography, and socrose density gradient ultracentrifugation. The enxyme had a molecular weight of approximately 35000 with a pH optimum of 8-8.5. Activity was substantially enhanced by supplements of 2-mercaptoethanol and to a lesser extent by cysteine-HCl or reduced gluthatione. No degradation of the enzyme into smaller subunits was observed when treated with 2-mercaptoethanol.

UI	MeSH Term	Description	Entries
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D002474	Cell-Free System	A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)	Cellfree System,Cell Free System,Cell-Free Systems,Cellfree Systems,System, Cell-Free,System, Cellfree,Systems, Cell-Free,Systems, Cellfree
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000150	Acinetobacter	A genus of gram-negative bacteria of the family MORAXELLACEAE, found in soil and water and of uncertain pathogenicity.	Herellea,Mima
D013379	Substrate Specificity	A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.	Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D013466	Sulfurtransferases	Enzymes which transfer sulfur atoms to various acceptor molecules. EC 2.8.1.	Sulfurtransferase
D013696	Temperature	The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.	Temperatures
D013884	Thiosulfate Sulfurtransferase	An enzyme that catalyzes the transfer of the planetary sulfur atom of thiosulfate ion to cyanide ion to form thiocyanate ion. EC 2.8.1.1.	Rhodanese,Thiosulfate Cyanide Transsulphurase,Thiosulfate Sulphurtransferase,Cyanide Transsulphurase, Thiosulfate,Sulfurtransferase, Thiosulfate,Sulphurtransferase, Thiosulfate,Transsulphurase, Thiosulfate Cyanide