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Using thin-layer chromatography fluorometry, gas chromatography mass spectrometry and field ionization mass spectrometry the findings of Green et al. regarding the identification of 5-methoxytryptamine in rat hypothalamus could not be confirmed. On derivatization using pentafluoropropionic anhydride, melatonin underwent partial transacylation to yield 5-methoxytryptamine as one of the by-products. We thus consider the finding of 5-methoxytryptamine in rat hypothalamus by mass fragmentography as an artifact forming from melatonin, which is normally present in rat hypothalamus.
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