Biomaterial Database

The effect of fluoride on ameloblasts of mouse molar tooth germs "in vitro". 1980

G E Levenson

Two day-old mouse first molar tooth germs were grown in medium containing sodium fluoride for up to 4 days. Dose levels ranged from 200 ppm fluoride down to 0.1 ppm. A dose of 100 ppm was lethal to tooth germs. Doses from 50 to 5 ppm resulted in consistent production of chains of large clear vacuoles in the supranuclear region of secretory ameloblasts together with large dark staining globules in the apical one third to one half of these cells. Enamel matrix secretion was arrested within this dose range as evidenced by the presence of only a very thin layer of enamel matrix in sections as compared with a thicker layer in similar areas of controls. Presecretory ameloblasts were not visibly affected by fluoride. Some variations in these effects were observed at 2 and 1 ppm fluoride. Tooth germs treated with 10 ppm for 48 hours, then grown in control medium for 4 days, underwent reversal of characteristic fluoride effects with resumption of enamel secretion. One ppm appeared to be the lower limit for production of these effects as observed with the light microscope. The cytology of fluorotic ameloblasts agreed with that of in vivo fluoride studies. Indications are that this in vitro system is promising for study of fluoride effects on developing teeth. Its advantages are discussed.

UI	MeSH Term	Description	Entries
D008963	Molar	The most posterior teeth on either side of the jaw, totaling eight in the deciduous dentition (2 on each side, upper and lower), and usually 12 in the permanent dentition (three on each side, upper and lower). They are grinding teeth, having large crowns and broad chewing surfaces. (Jablonski, Dictionary of Dentistry, 1992, p821)	Molars
D005459	Fluorides	Inorganic salts of hydrofluoric acid, HF, in which the fluorine atom is in the -1 oxidation state. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Sodium and stannous salts are commonly used in dentifrices.	Fluoride
D000565	Ameloblasts	Cylindrical epithelial cells in the innermost layer of the ENAMEL ORGAN. Their functions include contribution to the development of the dentinoenamel junction by the deposition of a layer of the matrix, thus producing the foundation for the prisms (the structural units of the DENTAL ENAMEL), and production of the matrix for the enamel prisms and interprismatic substance. (From Jablonski's Dictionary of Dentistry, 1992)	Ameloblast
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D014083	Tooth Germ	The collective tissues from which an entire tooth is formed, including the DENTAL SAC; ENAMEL ORGAN; and DENTAL PAPILLA. (From Jablonski, Dictionary of Dentistry, 1992)	Germ, Tooth,Germs, Tooth,Tooth Germs
D014617	Vacuoles	Any spaces or cavities within a cell. They may function in digestion, storage, secretion, or excretion.	Vacuole
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D066298	In Vitro Techniques	Methods to study reactions or processes taking place in an artificial environment outside the living organism.	In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In