-transparent.png){kind=logo}
Developmental changes in cell surface and cytoskeletal elements have been studied in human promyelocytic leukemia cels (line HL-60) which differentiate into functionally mature myeloid cells when grown in dimethyl sulfoxide (DMSO)-supplemented medium. Both differentiated and undifferentiated HL-60 cells bind fluorescent concanavalin A (F-Con A) in a diffuse pattern over the entire cell surface. As with normal neutrophils, pretreatment of the differentiated HL-60 cells with colchicine before incubation with Con A causes the formation of large cytoplasmic protrusions over which the lectin associates into a cap. On the other hand, similarly treated undifferentiated HL-60 cells do not form the cytoplasmic protuberances and are unable to cap the Con A. Transmission electron microscopy reveals that the number and distribution of microtubules and microfilaments change during differentiation. Thus, developing myeloid cells undergo important alterations in the structure and function of the cytoskeleton as they differentiate into mature phagocytes.
W J Brown, and
C F Norwood, and
R G Smith, and
W J Snell
November 2001,
Chemical research in toxicology,
W J Brown, and
C F Norwood, and
R G Smith, and
W J Snell
March 1985,
Shi yan sheng wu xue bao,
W J Brown, and
C F Norwood, and
R G Smith, and
W J Snell
October 1980,
Cancer letters,
W J Brown, and
C F Norwood, and
R G Smith, and
W J Snell
April 1990,
FEBS letters,
W J Brown, and
C F Norwood, and
R G Smith, and
W J Snell
October 1995,
Chemical & pharmaceutical bulletin,
W J Brown, and
C F Norwood, and
R G Smith, and
W J Snell
November 2011,
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica,
W J Brown, and
C F Norwood, and
R G Smith, and
W J Snell
June 1983,
Cancer research,
W J Brown, and
C F Norwood, and
R G Smith, and
W J Snell
December 1999,
Biological & pharmaceutical bulletin,
W J Brown, and
C F Norwood, and
R G Smith, and
W J Snell
January 1997,
Advances in experimental medicine and biology,
W J Brown, and
C F Norwood, and
R G Smith, and
W J Snell
January 1988,
Biomedica biochimica acta,
