BIOMAT Database Logo BIOMAT Database Logo

Immunodiagnosis of hepatitis B with high-affinity IgM monoclonal antibodies. 1981

J R Wands, and R I Carlson, and H Schoemaker, and K J Isselbacher, and V R Zurawski

High-affinity monoclonal IgG and IgM antibodies to hepatitis B surface antigen (HBsAg) have been prepared and their functional capabilities explored by means of solid-phase radioimmunoassays. 125I-labeled HBsAg binding studies indicated that monoclonal IgM antibodies against HBsAg (anti-HBs) coupled to a solid-phase support quantitatively bound more HbsAg at a faster rate than conventionally prepared anti-HBs reagents or other high-affinity IgG monoclonal anti-HBs antibodies. Consequently, IgM anti-HBs was also radiolabeled, and an IgM-IgM radioimmunoassay was developed for the immunodiagnosis of hepatitis B. The lower limit of this assay was approximately 100 pg +/- 30 (SEM) of HBsAg per ml of serum. Compared to available commercial radioassays, preliminary studies have shown the IgM-IgM assay to have increased sensitivity, which improved the detection of a HBsAg-associated determinant in acute hepatitis and post transfusion hepatitis. It is probable that the multivalent interaction between monoclonal IgM anti-HBs and the polydeterminant HBsAg is important in augmenting the performance of this monoclonal assay.

UI MeSH Term Description Entries
D007075 Immunoglobulin M A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally was called a macroglobulin. Gamma Globulin, 19S,IgM,IgM Antibody,IgM1,IgM2,19S Gamma Globulin,Antibody, IgM
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D011863 Radioimmunoassay Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation. Radioimmunoassays
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D006509 Hepatitis B INFLAMMATION of the LIVER in humans caused by a member of the ORTHOHEPADNAVIRUS genus, HEPATITIS B VIRUS. It is primarily transmitted by parenteral exposure, such as transfusion of contaminated blood or blood products, but can also be transmitted via sexual or intimate personal contact. Hepatitis B Virus Infection
D006514 Hepatitis B Surface Antigens Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen. Australia Antigen,HBsAg,Hepatitis B Surface Antigen,Antigen, Australia
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006822 Hybrid Cells Any cell, other than a ZYGOTE, that contains elements (such as NUCLEI and CYTOPLASM) from two or more different cells, usually produced by artificial CELL FUSION. Somatic Cell Hybrids,Cell Hybrid, Somatic,Cell Hybrids, Somatic,Cell, Hybrid,Cells, Hybrid,Hybrid Cell,Hybrid, Somatic Cell,Hybrids, Somatic Cell,Somatic Cell Hybrid
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus

Related Publications

J R Wands, and R I Carlson, and H Schoemaker, and K J Isselbacher, and V R Zurawski
High affinity human monoclonal antibodies directed against hepatitis B surface antigen.
July 1987, Journal of virological methods,
J R Wands, and R I Carlson, and H Schoemaker, and K J Isselbacher, and V R Zurawski
High affinity monoclonal antibodies to hepatitis B surface antigen (HBsAg) produced by somatic cell hybrids.
February 1981, Gastroenterology,
J R Wands, and R I Carlson, and H Schoemaker, and K J Isselbacher, and V R Zurawski
Characterization of high-affinity antifluorescyl IgM antibodies.
October 1981, Molecular immunology,
J R Wands, and R I Carlson, and H Schoemaker, and K J Isselbacher, and V R Zurawski
Production of IgM-monoclonal antibodies against hepatitis B surface antigen by hybridoma technique.
July 1987, Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology,
J R Wands, and R I Carlson, and H Schoemaker, and K J Isselbacher, and V R Zurawski
Monoclonal antibodies with high affinity for spiroperidol.
April 1988, Journal of neurochemistry,
J R Wands, and R I Carlson, and H Schoemaker, and K J Isselbacher, and V R Zurawski
The immunodiagnosis of lung cancer with monoclonal antibodies.
September 2005, Medical science monitor : international medical journal of experimental and clinical research,
J R Wands, and R I Carlson, and H Schoemaker, and K J Isselbacher, and V R Zurawski
Angiotensin II measurement with high-affinity monoclonal antibodies.
December 1988, Journal of hypertension. Supplement : official journal of the International Society of Hypertension,
J R Wands, and R I Carlson, and H Schoemaker, and K J Isselbacher, and V R Zurawski
Monoclonal antibodies for immunodiagnosis of human hydatidosis.
January 1987, Parasitology today (Personal ed.),
J R Wands, and R I Carlson, and H Schoemaker, and K J Isselbacher, and V R Zurawski
Monoclonal antibodies and immunodiagnosis of leukemia/lymphoma.
December 1985, Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society,
J R Wands, and R I Carlson, and H Schoemaker, and K J Isselbacher, and V R Zurawski
Restriction in IgM expression. IV. Affinity analysis of monoclonal anti-phosphorylcholine antibodies.
January 1983, Journal of immunology (Baltimore, Md. : 1950),
Copied contents to your clipboard!