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3 beta-hydroxysteroid dehydrogenase (3 beta-HDS) activity was demonstrated ultrastructurally in human corpora lutea obtained from the women aged 32-46 who were treated by the hysterectomy for various gynecological indications. The corpora lutea were removed from ovaries and cut into small pieces of 1 mm3. Specimens were incubated at 25 degrees C for 20 min. in the media containing dehydroepiandrosterone 0.6 mg, 0.1 M sodium citrate 0.3 ml, 5 mM potassium ferricyanide 1 ml, 30 mM copper sulfate 1 ml, N.A.D. 3.6 mg, sucrose 1.0 g and 0.1 M phosphate buffer (pH 7.2) 6 ml. For a control study, 1.5 mg cyanoketone (by the courtesy of Dr G.O. Pott), the specific inhibitor for 3 beta-HSD, was added to the media. The materials were fixed in 4% glutaraldhyde and 1% OsO4 and embedded in Epon 812. Electron dense reaction products of copper ferrocyanide were observed in smooth endoplasmic reticulum (sER) and in intercristal and outer space of mitochondria. In control studies, 3 beta-HSD activity were completely abolished. It is concluded that not only sER but also mitochondria has 3 beta-HSD activity in human corpora lutea. Furthermore, mitochondria seems to have capability to synthetize progesterone from cholesterol by itself.
A Takenaka, and
H Okamura, and
Y Okuda, and
K Morimoto, and
C G Huang, and
T Nishimura
July 1981,
Acta obstetrica et gynaecologica Japonica,
A Takenaka, and
H Okamura, and
Y Okuda, and
K Morimoto, and
C G Huang, and
T Nishimura
January 1987,
Journal of animal science,
A Takenaka, and
H Okamura, and
Y Okuda, and
K Morimoto, and
C G Huang, and
T Nishimura
July 1966,
Biochimica et biophysica acta,
A Takenaka, and
H Okamura, and
Y Okuda, and
K Morimoto, and
C G Huang, and
T Nishimura
April 2005,
Reproductive biology and endocrinology : RB&E,
A Takenaka, and
H Okamura, and
Y Okuda, and
K Morimoto, and
C G Huang, and
T Nishimura
April 1976,
Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles,
A Takenaka, and
H Okamura, and
Y Okuda, and
K Morimoto, and
C G Huang, and
T Nishimura
January 1981,
Journal de gynecologie, obstetrique et biologie de la reproduction,
A Takenaka, and
H Okamura, and
Y Okuda, and
K Morimoto, and
C G Huang, and
T Nishimura
January 1966,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
A Takenaka, and
H Okamura, and
Y Okuda, and
K Morimoto, and
C G Huang, and
T Nishimura
March 1990,
The Biochemical journal,
A Takenaka, and
H Okamura, and
Y Okuda, and
K Morimoto, and
C G Huang, and
T Nishimura
March 1965,
Acta endocrinologica,
A Takenaka, and
H Okamura, and
Y Okuda, and
K Morimoto, and
C G Huang, and
T Nishimura
November 1981,
Nihon Sanka Fujinka Gakkai zasshi,
