Biomaterial Database

Evaluation of a simplified filtration technique for the routine measurement of erythrocyte deformability. 1981

P F Leblond, and L Coulombe

The purpose of this study was to assess the value of a widely-used simplified filtration technique (mini-test) by comparing it, in parallel experiments, with a constant-flow rate method currently used in our laboratory for the measurement of erythrocyte deformability. Both methods employed 3 micrometer pore-diameter Nucleopore membranes and were performed under different flow conditions, using washed erythrocyte suspensions. In a total of 49 evaluable test pairs obtained from blood samples of 55 patients with either diabetes mellitus [36], post-splenectomy status [13] or haemolytic anaemia [6], we observed a sensitivity of 40% and a specificity of 75.5% for the mini-test versus our reference method. A near 20-fold lower cell/pore ratio appears as the single most important technical difference explaining the apparent lack of sensitivity of the mini-test. Suggestions are given as to how to modify the mini-test in order to obtain maximum sensitivity in the future.

UI	MeSH Term	Description	Entries
D008833	Microcirculation	The circulation of the BLOOD through the MICROVASCULAR NETWORK.	Microvascular Blood Flow,Microvascular Circulation,Blood Flow, Microvascular,Circulation, Microvascular,Flow, Microvascular Blood,Microvascular Blood Flows,Microvascular Circulations
D004910	Erythrocyte Membrane	The semi-permeable outer structure of a red blood cell. It is known as a red cell 'ghost' after HEMOLYSIS.	Erythrocyte Ghost,Red Cell Cytoskeleton,Red Cell Ghost,Erythrocyte Cytoskeleton,Cytoskeleton, Erythrocyte,Cytoskeleton, Red Cell,Erythrocyte Cytoskeletons,Erythrocyte Ghosts,Erythrocyte Membranes,Ghost, Erythrocyte,Ghost, Red Cell,Membrane, Erythrocyte,Red Cell Cytoskeletons,Red Cell Ghosts
D004912	Erythrocytes	Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.	Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014462	Ultrafiltration	The separation of particles from a suspension by passage through a filter with very fine pores. In ultrafiltration the separation is accomplished by convective transport; in DIALYSIS separation relies instead upon differential diffusion. Ultrafiltration occurs naturally and is a laboratory procedure. Artificial ultrafiltration of the blood is referred to as HEMOFILTRATION or HEMODIAFILTRATION (if combined with HEMODIALYSIS).