Biomaterial Database

Modification of carboxyl groups in Geotrichum candidum lipase. 1982

A Sugihara, and M Iwai, and Y Tsujisaka

Lipase from Geotrichum (Geo.) candidum was rapidly inactivated by incubation with water-soluble carbodiimide, 1-ethyl-3-dimethylaminopropyl carbodiimide (EDC), or 1-cyclohexyl-3-(2-morpholinyl-(4)-ethyl) carbodiimide metho-p-toluenesulfonate (CMC), at pH 4.8. The pH dependence of the rate of inactivation was consistent with the modification of carboxyl groups in the lipase. Reaction of the lipase with EDC in the presence of the nucleophile taurine showed that about 9 carboxyl groups per molecule of enzyme were modified with concomitant total loss of activity. This number was reduced to 4 when CMC was used as a carbodiimide instead of EDC. The modification had no effect on the CD spectrum in the ultraviolet region. Kinetic analysis of the effect of CMC on the lipase indicated that at least 1 CMC molecule bound to the enzyme during inactivation.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008049	Lipase	An enzyme of the hydrolase class that catalyzes the reaction of triacylglycerol and water to yield diacylglycerol and a fatty acid anion. It is produced by glands on the tongue and by the pancreas and initiates the digestion of dietary fats. (From Dorland, 27th ed) EC 3.1.1.3.	Triacylglycerol Lipase,Tributyrinase,Triglyceride Lipase,Acid Lipase,Acid Lipase A,Acid Lipase B,Acid Lipase I,Acid Lipase II,Exolipase,Monoester Lipase,Triacylglycerol Hydrolase,Triglyceridase,Triolean Hydrolase,Hydrolase, Triacylglycerol,Hydrolase, Triolean,Lipase A, Acid,Lipase B, Acid,Lipase I, Acid,Lipase II, Acid,Lipase, Acid,Lipase, Monoester,Lipase, Triglyceride
D002621	Chemistry	A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
D003028	CME-Carbodiimide		4-(2-((Cyclohexylimidocarbonyl)amino)ethyl)-4-methylmorpholinium,CME Carbodiimide,CMEC,Carbodiimide, CME
D003904	Mitosporic Fungi	A large and heterogenous group of fungi whose common characteristic is the absence of a sexual state. Many of the pathogenic fungi in humans belong to this group.	Deuteromycetes,Deuteromycota,Fungi imperfecti,Fungi, Mitosporic,Hyphomycetes,Deuteromycete,Deuteromycotas,Fungi imperfectus,Fungus, Mitosporic,Hyphomycete,Mitosporic Fungus,imperfectus, Fungi
D005022	Ethyldimethylaminopropyl Carbodiimide	Carbodiimide cross-linking reagent.	1-Ethyl-3-(3-Dimethylaminopropyl)Carbodiimide,3-(3-Dimethylaminopropyl)-1-Ethylcarbodiimide,EDAP-Carbodiimide,Carbodiimide, Ethyldimethylaminopropyl,EDAP Carbodiimide
D005848	Geotrichum	A mitosporic Saccharomycetales fungal genus, various species of which have been isolated from pulmonary lesions. Teleomorphs include Dipodascus and Galactomyces.	Geotrichums
D055598	Chemical Phenomena	The composition, structure, conformation, and properties of atoms and molecules, and their reaction and interaction processes.	Chemical Concepts,Chemical Processes,Physical Chemistry Concepts,Physical Chemistry Processes,Physicochemical Concepts,Physicochemical Phenomena,Physicochemical Processes,Chemical Phenomenon,Chemical Process,Physical Chemistry Phenomena,Physical Chemistry Process,Physicochemical Phenomenon,Physicochemical Process,Chemical Concept,Chemistry Process, Physical,Chemistry Processes, Physical,Concept, Chemical,Concept, Physical Chemistry,Concept, Physicochemical,Concepts, Chemical,Concepts, Physical Chemistry,Concepts, Physicochemical,Phenomena, Chemical,Phenomena, Physical Chemistry,Phenomena, Physicochemical,Phenomenon, Chemical,Phenomenon, Physicochemical,Physical Chemistry Concept,Physicochemical Concept,Process, Chemical,Process, Physical Chemistry,Process, Physicochemical,Processes, Chemical,Processes, Physical Chemistry,Processes, Physicochemical