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The histologic features of epithelial outgrowths from transplants of human buccal mucosa in nude mice are described. Nine-six samples of normal buccal mucosa were transplanted to subcutaneous sites in the flank region of nude mice. The tissue specimens were maintained in the mice for periods ranging from 1 to 67 d and were protected from overlying murine cutaneous and subcutaneous tissue by polyethylene capsules or by Millipore filters. The epithelial outgrowth from the transplants were formed over murine connective tissue, with and without substantial infiltrate of neutrophils, and over Millipore filter with and without interjacent murine connective tissue. The stratified squamous epithelium was essentially unkeratinized and thinner than that overlying originally transplanted connective tissue. The histologic features of the outgrowths were similar irrespective of the technique used or the type of underlying tissue.
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