Two citric acid-producing strains of the mould Aspergillus niger, cultivated on different sporulation media recommended and well-tried for Aspergilli, showed only poor formation of conidia the harvesting of which was difficult. Starting with a sporulation medium of MOYER et al. and substituting some of the constituents and determining their optimum concentrations, two semi-synthetic media were developed, containing glycerol and/or citric acid. On these sporulation media the two strains exhibited excellent growth and formation of a dense and regular carpet of conidia which could be easily harvested. Attention was payed to the significance of the cultivation temperature. The original biochemical properties of the two strains were not altered.