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Low concentrations (0.5-1 microM) of cytochalasins inhibit the thrombin-stimulated polymerization of monomeric actin to filamentous actin in platelets. Similar concentrations of cytochalasin B inhibit the formation and metabolism of arachidonic acid in horse platelets stimulated by low concentrations of thrombin (0.1-0.5 unit/ml). However, the release of serotonin is not inhibited by cytochalasin B. Cytochalasins B and D (0.5-1 microM) markedly reduce, in thrombin-stimulated human or horse platelets, the metabolism of the liberated arachidonic acid by cyclooxygenase activity to thromboxane B2 and 12-hydroxy-5,8,10-heptadecatrienoic acid and the conversion of arachidonic acid by lipoxygenase activity to 12-hydroxy-5,8,10,14-icosatetraenoic acid. The generation of arachidonic acid from platelet phospholipids and the formation of phosphatidic acid are much less affected by cytochalasin B or D. Cytochalasins do not directly inhibit platelet cyclooxygenase, lipoxygenase, phospholipase A2, or phosphatidyl-inositol-specific phospholipase C. In addition, the metabolism of exogenously added arachidonic acid by intact platelets is not inhibited by cytochalasins B and D. The results indicate that polymerization of actin in platelets stimulated by thrombin may be required for the effective metabolism of arachidonic acid released from platelet phospholipids.
W Siess, and
E G Lapetina, and
P Cuatrecasas
February 1990,
Biochemistry and cell biology = Biochimie et biologie cellulaire,
W Siess, and
E G Lapetina, and
P Cuatrecasas
September 1988,
Biochemical pharmacology,
W Siess, and
E G Lapetina, and
P Cuatrecasas
December 1989,
Biochemical and biophysical research communications,
W Siess, and
E G Lapetina, and
P Cuatrecasas
July 1985,
Rinsho byori. The Japanese journal of clinical pathology,
W Siess, and
E G Lapetina, and
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December 1987,
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W Siess, and
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July 1985,
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W Siess, and
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June 1984,
Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire,
W Siess, and
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August 1994,
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W Siess, and
E G Lapetina, and
P Cuatrecasas
February 1986,
Biochemical and biophysical research communications,
W Siess, and
E G Lapetina, and
P Cuatrecasas
August 2017,
BMC complementary and alternative medicine,
