Biomaterial Database

Minimum nutritional requirements for cellular growth and extracellular slime polysaccharide production by the human dental plaque bacterial mutant Actinomyces viscosus T14Av. 1982

T Ooshima, and H K Kuramitsu

The requirements were examined in chemically-defined media. Magnesium sulphate, potassium phosphate and adenine were essential for cellular growth and sodium bicarbonate and inositol further stimulated growth. The addition of sodium phosphate to this medium stimulated both cellular growth and slime production. In contrast, ammonium sulphate inhibited slime production. Biotin and thiamine stimulated cellular growth and nicotinic acid was essential for slime production. Glutamine, alanine and cystine were essential for cellular growth. A minimal medium which supported both good cellular growth and slime production for strain T14Av contained adenine, sodium bicarbonate, MgSO4, inositol, glucose, KH2PO4, Na2HPO4, biotin, thiamine, nicotinic acid, arginine, glutamine, alanine and cystine.

UI	MeSH Term	Description	Entries
D010710	Phosphates	Inorganic salts of phosphoric acid.	Inorganic Phosphate,Phosphates, Inorganic,Inorganic Phosphates,Orthophosphate,Phosphate,Phosphate, Inorganic
D011135	Polysaccharides, Bacterial	Polysaccharides found in bacteria and in capsules thereof.	Bacterial Polysaccharides
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D003773	Dental Plaque	A film that attaches to teeth, often causing DENTAL CARIES and GINGIVITIS. It is composed of MUCINS, secreted from salivary glands, and microorganisms.	Plaque, Dental
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000190	Actinomyces	A genus of gram-positive, rod-shaped bacteria whose organisms are nonmotile. Filaments that may be present in certain species are either straight or wavy and may have swollen or clubbed heads.
D000596	Amino Acids	Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.	Amino Acid,Acid, Amino,Acids, Amino
D013431	Sulfates	Inorganic salts of sulfuric acid.	Sulfate,Sulfates, Inorganic,Inorganic Sulfates
D014815	Vitamins	Organic substances that are required in small amounts for maintenance and growth, but which cannot be manufactured by the human body.	Vitamin