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In order to investigate the cytoprotective action of 16-16-dimethyl-prostaglandin E2 (16-16D), we studied its effect on sacs of isolated amphibian gastric mucosa known to ulcerate with high frequency in the absence of nutrient HCO3-. The actions of 16-16D, 2.85 X 10(-6) M, were also studied in an in vitro chamber. The incidence of ulceration in HCO3- -free nutrient media containing 10(-4) M histamine was significantly reduced by 16-16D. When artificial gastric juice (AGJ) was instilled into the lumen of the sac, protection occurred only after a pretreatment period of 60 min. Cytoprotection was not observed when active secretion of H+ was inhibited with 10(-3) M metiamide, and AGJ was placed in the lumen of the sac. In open sheets of fundus stimulated with 10(-4) M histamine, 16-16D did not influence H+ secretion. Isc was significantly higher than in control tissues. We conclude that 16-16D is cytoprotective in amphibian gastric mucosa by an action independent of changes in acid secretion or in blood flow. In addition, our studies suggest that 16-16D may increase the availability to the surface cells of nutrient HCO3- produced by actively secreting oxyntic cells.
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R Schiessel, and
E Kivilaakso, and
J B Matthews, and
L A Fleischer, and
G Bartzokis, and
W Silen
March 1997,
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A Barzilai, and
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J B Matthews, and
L A Fleischer, and
G Bartzokis, and
W Silen
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A Barzilai, and
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E Kivilaakso, and
J B Matthews, and
L A Fleischer, and
G Bartzokis, and
W Silen
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Digestive diseases and sciences,
A Barzilai, and
R Schiessel, and
E Kivilaakso, and
J B Matthews, and
L A Fleischer, and
G Bartzokis, and
W Silen
January 1981,
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A Barzilai, and
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G Bartzokis, and
W Silen
September 1975,
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A Barzilai, and
R Schiessel, and
E Kivilaakso, and
J B Matthews, and
L A Fleischer, and
G Bartzokis, and
W Silen
December 1981,
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A Barzilai, and
R Schiessel, and
E Kivilaakso, and
J B Matthews, and
L A Fleischer, and
G Bartzokis, and
W Silen
April 1982,
Gastroenterology,
A Barzilai, and
R Schiessel, and
E Kivilaakso, and
J B Matthews, and
L A Fleischer, and
G Bartzokis, and
W Silen
August 1981,
Surgery,
A Barzilai, and
R Schiessel, and
E Kivilaakso, and
J B Matthews, and
L A Fleischer, and
G Bartzokis, and
W Silen
January 1985,
Gastroenterology,
A Barzilai, and
R Schiessel, and
E Kivilaakso, and
J B Matthews, and
L A Fleischer, and
G Bartzokis, and
W Silen
July 1973,
Prostaglandins,
