A simple assay for uridine diphosphate galactose 4-epimerase activity. 1980

G Vaca, and J Sanchez-Corona, and N Olivares, and C Medina, and B Ibarra, and J M Cantu

A simple fluorescent test for the activity of blood uridine diphosphate galactose 4-epimerase which converts uridine diphosphate galactose to uridine diphosphate glucose is described. The enzyme activity is visually estimated by to reduction of NAD+ (non fluorescent) to NADH (fluorescent) in a coupled reaction with uridine diphosphate glucose dehydrogenase. The appearance of fluorescence indicates that epimerase activity is present in the sample. The usefulness of this test in newborn screening programs for inborn errors of galactose metabolism is stressed.

UI MeSH Term Description Entries
D007231 Infant, Newborn An infant during the first 28 days after birth. Neonate,Newborns,Infants, Newborn,Neonates,Newborn,Newborn Infant,Newborn Infants
D008722 Methods A series of steps taken in order to conduct research. Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D002238 Carbohydrate Epimerases Enzymes that catalyze the epimerization of chiral centers within carbohydrates or their derivatives. EC 5.1.3. Carbohydrate Isomerases,Epimerases, Carbohydrate,Isomerases, Carbohydrate
D005453 Fluorescence The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014534 UDPglucose 4-Epimerase A necessary enzyme in the metabolism of galactose. It reversibly catalyzes the conversion of UDPglucose to UDPgalactose. NAD+ is an essential component for enzymatic activity. EC 5.1.3.2. UDP Galactose Epimerase,UDP Glucose Epimerase,UDPgalactose 4-Epimerase,Uridine Diphosphate Glucose Epimerase,UDP-Galactose 4-Epimerase,UDP-Glucose 4-Epimerase,4-Epimerase, UDP-Galactose,4-Epimerase, UDP-Glucose,4-Epimerase, UDPgalactose,4-Epimerase, UDPglucose,Epimerase, UDP Galactose,Epimerase, UDP Glucose,Galactose Epimerase, UDP,Glucose Epimerase, UDP,UDP Galactose 4 Epimerase,UDP Glucose 4 Epimerase,UDPgalactose 4 Epimerase,UDPglucose 4 Epimerase

Related Publications

G Vaca, and J Sanchez-Corona, and N Olivares, and C Medina, and B Ibarra, and J M Cantu
October 1980, American journal of diseases of children (1960),
G Vaca, and J Sanchez-Corona, and N Olivares, and C Medina, and B Ibarra, and J M Cantu
February 1981, European journal of pediatrics,
G Vaca, and J Sanchez-Corona, and N Olivares, and C Medina, and B Ibarra, and J M Cantu
January 1983, Prenatal diagnosis,
G Vaca, and J Sanchez-Corona, and N Olivares, and C Medina, and B Ibarra, and J M Cantu
April 1999, Archives of disease in childhood,
G Vaca, and J Sanchez-Corona, and N Olivares, and C Medina, and B Ibarra, and J M Cantu
January 1972, Biochemistry,
G Vaca, and J Sanchez-Corona, and N Olivares, and C Medina, and B Ibarra, and J M Cantu
November 1971, Proceedings of the National Academy of Sciences of the United States of America,
G Vaca, and J Sanchez-Corona, and N Olivares, and C Medina, and B Ibarra, and J M Cantu
February 1973, The Biochemical journal,
G Vaca, and J Sanchez-Corona, and N Olivares, and C Medina, and B Ibarra, and J M Cantu
October 1995, The Journal of pediatrics,
G Vaca, and J Sanchez-Corona, and N Olivares, and C Medina, and B Ibarra, and J M Cantu
April 1971, Biochemistry,
G Vaca, and J Sanchez-Corona, and N Olivares, and C Medina, and B Ibarra, and J M Cantu
March 1990, Biochemistry,
Copied contents to your clipboard!