BIOMAT Database Logo BIOMAT Database Logo

Mouse alloantibodies capable of blocking cytotoxic T cell function. II. Further study on the relationship between the blocking antibodies and the products of the Lyt-2 locus. 1980

N Shinohara, and U Hämmerling, and D H Sachs

The specificity of mouse alloantibodies which blocked allogeneic killer cells has been confirmed as Ly-2.2 using B6 X C3H recombinant inbred (RI) strains. All of the RI strains tested were sensitive to the cell-mediated lymphocytotoxicity (CML)-blocking effect of our C3H anti-B 10.BR antisera and also carried the Lyt-2b allele. This result and studies using Lyt-2-congenic strains firmly localized the genes encoding the target antigen of CML-blocking antibodies in or near the Lyt-2 locus. In addition, a monoclonal anti-Lyt-2.2 antibody was found to block the CML reaction of B6 killer cells, but not that of B6 Ly-2.1 killers. Blocking antibodies to one allele blocked killing by Lyt-2-heterozygous F1 killer cells, but the inhibition curve reached a plateau at a significantly lower level than that seen on the killer cells of the sensitive parent. Our results thus suggest that Lyt-2 antigens are of functional significance on killer T cells, but do not reveal whether or not that significance involves the T cell receptor.

UI MeSH Term Description Entries
D007518 Isoantibodies Antibodies from an individual that react with ISOANTIGENS of another individual of the same species. Alloantibodies
D007519 Isoantigens Antigens that exist in alternative (allelic) forms in a single species. When an isoantigen is encountered by species members who lack it, an immune response is induced. Typical isoantigens are the BLOOD GROUP ANTIGENS. Alloantigens,Alloantigen,Isoantigen
D007694 Killer Cells, Natural Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type. NK Cells,Natural Killer Cells,Cell, NK,Cell, Natural Killer,Cells, NK,Cells, Natural Killer,Killer Cell, Natural,NK Cell,Natural Killer Cell
D008297 Male Males
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D008809 Mice, Inbred C3H An inbred strain of mouse that is used as a general purpose strain in a wide variety of RESEARCH areas including CANCER; INFECTIOUS DISEASES; sensorineural, and cardiovascular biology research. Mice, C3H,Mouse, C3H,Mouse, Inbred C3H,C3H Mice,C3H Mice, Inbred,C3H Mouse,C3H Mouse, Inbred,Inbred C3H Mice,Inbred C3H Mouse
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D002874 Chromosome Mapping Any method used for determining the location of and relative distances between genes on a chromosome. Gene Mapping,Linkage Mapping,Genome Mapping,Chromosome Mappings,Gene Mappings,Genome Mappings,Linkage Mappings,Mapping, Chromosome,Mapping, Gene,Mapping, Genome,Mapping, Linkage,Mappings, Chromosome,Mappings, Gene,Mappings, Genome,Mappings, Linkage
D003602 Cytotoxicity, Immunologic The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement. Tumoricidal Activity, Immunologic,Immunologic Cytotoxicity,Immunologic Tumoricidal Activities,Immunologic Tumoricidal Activity,Tumoricidal Activities, Immunologic
D005260 Female Females

Related Publications

N Shinohara, and U Hämmerling, and D H Sachs
Mouse alloantibodies capable of blocking cytotoxic T-cell function. I. Relationship between the antigen reactive with blocking antibodies and the Lyt-2 locus.
September 1979, The Journal of experimental medicine,
N Shinohara, and U Hämmerling, and D H Sachs
Mouse alloantibodies capable of blocking cytotoxic T cell function. III. Studies of the mechanism of blocking of CML by anti-Lyt-2 antibodies.
October 1981, Journal of immunology (Baltimore, Md. : 1950),
N Shinohara, and U Hämmerling, and D H Sachs
Mouse alloantibodies capable of blocking cytotoxic T cell function. IV. Comparative analysis of the blocking effects of anti-Lyt-2 and anti-H-2 antibodies on allogeneic and PHA-mediated killing.
January 1983, Microbiology and immunology,
N Shinohara, and U Hämmerling, and D H Sachs
Mouse alloantibodies capable of blocking cytotoxic T cell function. V. The majority of I region-specific CTL are Lyt-2+ but are relatively resistant to anti-Lyt-2 blocking.
February 1984, Journal of immunology (Baltimore, Md. : 1950),
N Shinohara, and U Hämmerling, and D H Sachs
Blocking effect of lyt-2 antibodies on T cell functions.
September 1980, The Journal of experimental medicine,
N Shinohara, and U Hämmerling, and D H Sachs
Role of CD8 (Lyt-2) in cytotoxic T-cell function. Analysis of variants of a cytotoxic T-cell clone with reduced Lyt-2 expression.
July 1988, Scandinavian journal of immunology,
N Shinohara, and U Hämmerling, and D H Sachs
Effects of anti-Lyt-2 and anti-L3T4 monoclonal antibodies on the function of cytotoxic T lymphocyte/helper T lymphocyte hybrid T cell clones.
September 1988, Journal of immunology (Baltimore, Md. : 1950),
N Shinohara, and U Hämmerling, and D H Sachs
Cytotoxic T cells: Lyt phenotype and blocking of killing activity by Lyt antisera.
April 1979, Proceedings of the National Academy of Sciences of the United States of America,
N Shinohara, and U Hämmerling, and D H Sachs
Mouse melanoma antigen recognized by Lyt-2- and L3T4- cytotoxic T-lymphocytes.
May 1988, Cancer research,
N Shinohara, and U Hämmerling, and D H Sachs
Characterization of interleukin 2-dependent cytotoxic T-cell clones: specificity, cell surface phenotype, and susceptibility to blocking by Lyt antisera.
February 1983, Cancer research,
Copied contents to your clipboard!