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Heterologous anti-delta-chain antibodies have an adjuvant effect on specific in vivo humoral immune responses to simultaneously, or subsequently, injected antigens in the rat and rhesus monkey. We have used a hybridoma-secreted antibody that binds murine delta-chain of the allotype (4.22aM delta a) to study this phenomenon in the mouse and to investigate the mechanism of this effect. Injection of 4.22aM delta a into BALB/c mice removes almost all surface IgD (sIgD) from splenic B lymphocites. sIgD does not reappear until the serum level of 4.22aM delta a decreased 5-7 d after injection. 4.22aM delta a fails to induce detectable proliferation or to raise total serum Ig levels substantially above control values. However, 4.22aM dalta a injected 24 h before antigen elicits an approximately twofold enhancement of serum IgM and a 3- to 10-fold enhancement of serum IgG anti-trintriphenyl (TNP) antibodies in response to immunization with optimal doses of TNP-Ficoll or TNP-sheep red blood cells (TNP-SRBC). 4.22aM delta a injected 1 wk before or 3 d after TNP-SRBC, however, has no effect on IgG anti-TNP levels. The adjuvant effect of anti-delta-chain antibody was markedly decreased when suboptimal antigen doses were used. Furthermore, even in the case of TNP-Ficoll, a relatively T-independent antigen, the ability of 4.22aM dalta a to enhance the anti-TNP antibody response was T cell dependent. Our data suggest that the binding of anti-delta-chain antibody to cell sIgD may partially activate B lymphocytes and make them more capable of differentiating into antibody-secreting cells when stimulated by antigen-specific T cell help.
F D Finkelham, and
V L Woods, and
S B Wilburn, and
J J Mond, and
K E Stein, and
A Berning, and
I Scher
December 2011,
International immunopharmacology,
F D Finkelham, and
V L Woods, and
S B Wilburn, and
J J Mond, and
K E Stein, and
A Berning, and
I Scher
August 1979,
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F D Finkelham, and
V L Woods, and
S B Wilburn, and
J J Mond, and
K E Stein, and
A Berning, and
I Scher
June 1986,
Arerugi = [Allergy],
F D Finkelham, and
V L Woods, and
S B Wilburn, and
J J Mond, and
K E Stein, and
A Berning, and
I Scher
October 1987,
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F D Finkelham, and
V L Woods, and
S B Wilburn, and
J J Mond, and
K E Stein, and
A Berning, and
I Scher
February 1996,
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F D Finkelham, and
V L Woods, and
S B Wilburn, and
J J Mond, and
K E Stein, and
A Berning, and
I Scher
November 1983,
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F D Finkelham, and
V L Woods, and
S B Wilburn, and
J J Mond, and
K E Stein, and
A Berning, and
I Scher
January 1991,
Journal of immunology (Baltimore, Md. : 1950),
F D Finkelham, and
V L Woods, and
S B Wilburn, and
J J Mond, and
K E Stein, and
A Berning, and
I Scher
February 1989,
Transplantation proceedings,
F D Finkelham, and
V L Woods, and
S B Wilburn, and
J J Mond, and
K E Stein, and
A Berning, and
I Scher
August 1969,
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F D Finkelham, and
V L Woods, and
S B Wilburn, and
J J Mond, and
K E Stein, and
A Berning, and
I Scher
October 1983,
Cellular immunology,
