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Cytocentrifuged preparations of glutaraldehyde-fixed E rosettes were stained for acid alpha-naphthyl-acetate esterase activity (ANAE). On average, 84% of the rosette-forming cells (E+) also stained positively for the enzyme activity (A+). ANAE staining of blood smears of thirty-three healthy controls was compared to E rosetting by a conventional method. A+ and E+ fractional values are highly significantly correlated; the regression of E+ on A+ is E+ = 0.805.A+ + 16.8 (P < 10(-6)). Since the correlation between E+ and A+ cells is both statistically and cytologically significant, we propose blood smear ANAE staining as a simple and convenient method to enumerate circulating T lymphocytes in clinical practice.
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August 1983,
American journal of veterinary research,
F A Ferrari, and
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Acta cytologica,
F A Ferrari, and
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F A Ferrari, and
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Chinese medical journal,
F A Ferrari, and
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V Burgio, and
A G Siccardi
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F A Ferrari, and
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V Burgio, and
A G Siccardi
January 1982,
American journal of primatology,
