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The lymphocytes that isolated from the both peripheral venous blood (PVB) and uterine venous blood (UVB) of pregnant women with a complication of myoma were studied on the subpopulation (E-rosette and S-Ig method) and lymphoblastoid transformation to mitogens. Two methods on the E-rosette in this study were applied to make a clear correlation between masking phenomenon and humoral factors. One is to be used lymphocytes that washed five times with P.B.S., the other one cultured in RPMI 1640 with 20% FCS for 72 hours afterward. The results were obtained as follows. 1) Although T-cell of UVB displayed significantly lower than that of PVB. The count of T-cell of both PVB and UVB elevated to standard level after washing five times with P.B.S. and/or cultured for 72 hours. 2) The average counts of B-cell by the S-Ig were not detected on the difference between of PVB and UVB. 3) No differences in ratio of lymphoblastoid were recognized on the both lymphocytes of PVB and UVB to PHA and PWM too. 4) AFter five times washing, the counts of T-cell in UVB improved to standard value range. Therefore no difference of the counts in T-cell were detected between in PVB and UVB.
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