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Hexokinase (ATP: D-hexose 6-phosphotransferase, EC 2.7.1.1) type 1 from human erythrocytes exists in four electrophoretical distinct forms, termed Ia, Ib, Ic and Id in order of their increasing anodal electrophoretic mobility at pH 8.8. We were able to separate type Ia, Ib and Icd on phosphocellulose by using a discontinuous gradient elution. The three chromatographically distinct forms do not differ in their affinity constants for the substrates glucose and MgATP2-. In addition the inhibition by glucose 1,6-diphosphate does not differ significantly for all forms. However, the regulation of these inhibitions by inorganic phosphate is much less for type Ia compared to the other subtypes (P = 0.001). Aging of the red cells is accompanied by a relative increase of the proportion of type Ic and Ia, which is the less regulated form of the enzyme. This shift in electrophoretic and regulatory properties is argued to be due to a post-translational modification of the primary enzyme.
G Rijksen, and
G Jansen, and
R J Kraaijenhagen, and
M J Van der Vlist, and
A M Vlug, and
G E Staal
September 1988,
The Biochemical journal,
G Rijksen, and
G Jansen, and
R J Kraaijenhagen, and
M J Van der Vlist, and
A M Vlug, and
G E Staal
October 1986,
Biochimica et biophysica acta,
G Rijksen, and
G Jansen, and
R J Kraaijenhagen, and
M J Van der Vlist, and
A M Vlug, and
G E Staal
December 1979,
Proceedings of the National Academy of Sciences of the United States of America,
G Rijksen, and
G Jansen, and
R J Kraaijenhagen, and
M J Van der Vlist, and
A M Vlug, and
G E Staal
January 1968,
Science (New York, N.Y.),
G Rijksen, and
G Jansen, and
R J Kraaijenhagen, and
M J Van der Vlist, and
A M Vlug, and
G E Staal
February 1968,
Science (New York, N.Y.),
G Rijksen, and
G Jansen, and
R J Kraaijenhagen, and
M J Van der Vlist, and
A M Vlug, and
G E Staal
February 1968,
Science (New York, N.Y.),
G Rijksen, and
G Jansen, and
R J Kraaijenhagen, and
M J Van der Vlist, and
A M Vlug, and
G E Staal
January 1988,
Archives of biochemistry and biophysics,
G Rijksen, and
G Jansen, and
R J Kraaijenhagen, and
M J Van der Vlist, and
A M Vlug, and
G E Staal
November 1966,
Nature,
G Rijksen, and
G Jansen, and
R J Kraaijenhagen, and
M J Van der Vlist, and
A M Vlug, and
G E Staal
November 1966,
Nature,
G Rijksen, and
G Jansen, and
R J Kraaijenhagen, and
M J Van der Vlist, and
A M Vlug, and
G E Staal
January 1992,
The International journal of biochemistry,
