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When [125I]iodoepidermal growth factor is incubated with freshly isolated rat hepatocytes, cell-associated radioactivity reaches apparent steady state by 60 min at 20 C and by 30 min of incubation at 37 C. When the distribution of cell-associated radioactivity is studied at different times of incubation by quantitative electron microscopic autoradiography, the ligand initially associates with the plasma membrane and is progressively internalized as a function of time. The internalized ligand preferentially associates with lysosome-like structures. Qualitatively, these events are similar to those previously obtained with labeled insulin and glucagon in this cell, but quantitatively, the internalization of epidermal growth factor is much greater. The data suggest that the ligand or its specific receptor rather than the cell type is the major determinant of the rate of internalization.
J L Carpentier, and
P Gorden, and
P Freychet, and
B Canivet, and
L Orci
August 1986,
Journal of cell science,
J L Carpentier, and
P Gorden, and
P Freychet, and
B Canivet, and
L Orci
January 1969,
Zeitschrift fur Zellforschung und mikroskopische Anatomie (Vienna, Austria : 1948),
J L Carpentier, and
P Gorden, and
P Freychet, and
B Canivet, and
L Orci
April 1984,
Experimental cell research,
J L Carpentier, and
P Gorden, and
P Freychet, and
B Canivet, and
L Orci
July 1993,
The Journal of comparative neurology,
J L Carpentier, and
P Gorden, and
P Freychet, and
B Canivet, and
L Orci
January 1993,
In vivo (Athens, Greece),
J L Carpentier, and
P Gorden, and
P Freychet, and
B Canivet, and
L Orci
August 1992,
The Journal of experimental zoology,
J L Carpentier, and
P Gorden, and
P Freychet, and
B Canivet, and
L Orci
November 1996,
Synapse (New York, N.Y.),
J L Carpentier, and
P Gorden, and
P Freychet, and
B Canivet, and
L Orci
September 1985,
The American journal of anatomy,
J L Carpentier, and
P Gorden, and
P Freychet, and
B Canivet, and
L Orci
January 1982,
Arkhiv patologii,
J L Carpentier, and
P Gorden, and
P Freychet, and
B Canivet, and
L Orci
July 1977,
Journal of anatomy,
