Biomaterial Database

[The in vitro proliferative response of human peripheral blood lymphocytes stimulated with purified concanavalin A and the analysis of subpopulations of T-cells (author's transl)]. 1981

T Oh-Ishi

Human peripheral blood lymphocytes (PBL) were separated from anticoagulated peripheral blood using the Ficoll-Conraxin-L gradient technique, and were cultured with various concentrations of highly purified concanavalin A (Con A (F3)). Tritiated thymidine incorporation in these cultures was measured. PBL responded well to low doses of Con A (F3) and high doses of Con A (F3), and the dose-response curve to Con A (F3) of PBL seemed to show that bimodal proliferative response with a notch or a peak at 5--6 micrograms/ml of Con A (F3) and the other peak at the higher doses of Con A (F3). As it has been well known that E-rosette-forming cells (E-RFC) in human peripheral blood can be subdivided into two T-cell subpopulations on the basis of their relative affinity for sheep red blood cell (SRBC), we have examined how these T-cell subpopulations could responded to Con A (F3) at varying concentrations. We found that "Low-affinity E-RFC" could be optimally stimulated by Con A (F3) at 5--6 micrograms/ml, while "High-affinity E-RFC" at 8--12 micrograms/ml. 3H-thymidine incorporation of high-affinity E-RFC was strikingly increased at low doses of Con A (F3) when low-affinity E-RFC and high-affinity E-RFC were mixed at 25: 75 ratio, and the dose-response curve was very similar to that of PBL. Moreover when monocytes were added to high affinity E-RFC and the mixture was cultured, the doses-response curve was elevated at low doses of Con A (F3), but the dose-response curve was similar to that of high-affinity E-RFC and different from that of low-affinity E-RFC. Our results suggest that low-affinity E-RFC that has the capacity to respond optimally to Con A (F3) at 5--6 micrograms/ml in the in vitro proliferative response has the important effect upon the in vitro proliferative response of PBL to low doses of Con A (F3).

UI	MeSH Term	Description	Entries
D008213	Lymphocyte Activation	Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.	Blast Transformation,Blastogenesis,Lymphoblast Transformation,Lymphocyte Stimulation,Lymphocyte Transformation,Transformation, Blast,Transformation, Lymphoblast,Transformation, Lymphocyte,Activation, Lymphocyte,Stimulation, Lymphocyte
D008297	Male		Males
D003208	Concanavalin A	A MANNOSE/GLUCOSE binding lectin isolated from the jack bean (Canavalia ensiformis). It is a potent mitogen used to stimulate cell proliferation in lymphocytes, primarily T-lymphocyte, cultures.
D004305	Dose-Response Relationship, Drug	The relationship between the dose of an administered drug and the response of the organism to the drug.	Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000293	Adolescent	A person 13 to 18 years of age.	Adolescence,Youth,Adolescents,Adolescents, Female,Adolescents, Male,Teenagers,Teens,Adolescent, Female,Adolescent, Male,Female Adolescent,Female Adolescents,Male Adolescent,Male Adolescents,Teen,Teenager,Youths
D000328	Adult	A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available.	Adults
D012397	Rosette Formation	The in vitro formation of clusters consisting of a cell (usually a lymphocyte) surrounded by antigenic cells or antigen-bearing particles (usually erythrocytes, which may or may not be coated with antibody or antibody and complement). The rosette-forming cell may be an antibody-forming cell, a memory cell, a T-cell, a cell bearing surface cytophilic antibodies, or a monocyte possessing Fc receptors. Rosette formation can be used to identify specific populations of these cells.	Immunocytoadherence,Formation, Rosette,Formations, Rosette,Immunocytoadherences,Rosette Formations
D013601	T-Lymphocytes	Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.	T Cell,T Lymphocyte,T-Cells,Thymus-Dependent Lymphocytes,Cell, T,Cells, T,Lymphocyte, T,Lymphocyte, Thymus-Dependent,Lymphocytes, T,Lymphocytes, Thymus-Dependent,T Cells,T Lymphocytes,T-Cell,T-Lymphocyte,Thymus Dependent Lymphocytes,Thymus-Dependent Lymphocyte