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Intracellular deoxynucleoside triphosphate pools of normal bone marrow, thymocytes and cells from patients with ALL and AML were measured after 2 h incubation with deoxycoformycin (dCF) 10(-5) M and deoxyadenosine (AdR) 10(-4) M in vitro and after another 30 min incubation in the absence of dCF and AdR ('chase' experiment). Incubation with dCF and AdR resulted in a significant rise of dATP concentrations in all groups (the highest rises occurring in the leukaemic groups particularly in AML and Thy-ALL). The concentrations of the other three deoxyribonucleoside triphosphates fell in all groups. The dATP level fell during the 'chase' period but in Thy-ALL and thymocytes this fall was insignificant and slower than in the other groups. This suggests that not only intracellular build-up of dATP but also the capacity of the cell to degrade dATP is important for in vivo cytotoxicity of dCF treatment. These results help to explain the differences in response to dCF of the different leukaemias.
T Sylwestrowicz, and
A Piga, and
P Murphy, and
K Ganeshaguru, and
N H Russell, and
H G Prentice, and
A V Hoffbrand
October 1982,
Blood,
T Sylwestrowicz, and
A Piga, and
P Murphy, and
K Ganeshaguru, and
N H Russell, and
H G Prentice, and
A V Hoffbrand
February 1988,
Human immunology,
T Sylwestrowicz, and
A Piga, and
P Murphy, and
K Ganeshaguru, and
N H Russell, and
H G Prentice, and
A V Hoffbrand
July 1979,
Journal of immunology (Baltimore, Md. : 1950),
T Sylwestrowicz, and
A Piga, and
P Murphy, and
K Ganeshaguru, and
N H Russell, and
H G Prentice, and
A V Hoffbrand
December 1985,
The Tohoku journal of experimental medicine,
T Sylwestrowicz, and
A Piga, and
P Murphy, and
K Ganeshaguru, and
N H Russell, and
H G Prentice, and
A V Hoffbrand
October 2000,
Journal of cellular biochemistry,
T Sylwestrowicz, and
A Piga, and
P Murphy, and
K Ganeshaguru, and
N H Russell, and
H G Prentice, and
A V Hoffbrand
June 1990,
Journal of cellular biochemistry,
T Sylwestrowicz, and
A Piga, and
P Murphy, and
K Ganeshaguru, and
N H Russell, and
H G Prentice, and
A V Hoffbrand
August 1978,
Cancer research,
T Sylwestrowicz, and
A Piga, and
P Murphy, and
K Ganeshaguru, and
N H Russell, and
H G Prentice, and
A V Hoffbrand
February 1984,
Cancer research,
T Sylwestrowicz, and
A Piga, and
P Murphy, and
K Ganeshaguru, and
N H Russell, and
H G Prentice, and
A V Hoffbrand
April 1982,
Biochemical pharmacology,
T Sylwestrowicz, and
A Piga, and
P Murphy, and
K Ganeshaguru, and
N H Russell, and
H G Prentice, and
A V Hoffbrand
May 1988,
The Journal of biological chemistry,
