Biomaterial Database

Cholesterol-lipid interactions in membranes. The saturation concentration of cholesterol in bilayers of various lipids. 1978

H Reiber

1. The integration of cholesterol in a lipid bilayer can be visualized by changes in the fluorescence properties of the probe N-phenyl-1-naphthylamine (NPN). An increasing cholesterol content in the lipid phase corresponds to decreasing fluorescence intensity of NPN and a short wave shift of the emission spectrum. 2. Equilibrium constants for the partition of NPN between water and the various lipid phases are reported. An increasing cholesterol content in a bilayer decreases the solubility of NPN in the bilayer. 3. The saturation concentration of cholesterol in bilayers of various lipids prepared by ultrasonication is determined using the flourescence probe NPN. The maximal molar ratio of cholesterol : lipid is 2 : 1 for sphingomyelin or egg phosphatidylcholine and 1 : 1 for cerebroside, dipalmitoyl phosphatidylcholine, or dipalmitoyl phosphatidylethanolamine. 4. The comparison of the maximal molar ratio of cholesterol : lipid with the number of proton donor and proton acceptor sites in the lipid moiety is used for a discussion of the polar interactions of cholesterol within a lipid bilayer.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008567	Membranes, Artificial	Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION.	Artificial Membranes,Artificial Membrane,Membrane, Artificial
D010743	Phospholipids	Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.	Phosphatides,Phospholipid
D002784	Cholesterol	The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils.	Epicholesterol
D006017	Glycolipids	Any compound containing one or more monosaccharide residues bound by a glycosidic linkage to a hydrophobic moiety such as an acylglycerol (see GLYCERIDES), a sphingoid, a ceramide (CERAMIDES) (N-acylsphingoid) or a prenyl phosphate. (From IUPAC's webpage)	Glycolipid
D013050	Spectrometry, Fluorescence	Measurement of the intensity and quality of fluorescence.	Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D015057	1-Naphthylamine	A suspected industrial carcinogen (and listed as such by OSHA). Its N-hydroxy metabolite is strongly carcinogenic and mutagenic.	1-Aminonaphthalene,alpha-Naphthylamine,8-Aminonaphthalene,Naphthalidine,1 Aminonaphthalene,1 Naphthylamine,8 Aminonaphthalene,alpha Naphthylamine