Biomaterial Database

The effect of nutritional state on the lipoprotein lipase activity of isolated fat cells. 1978

I M Spencer, and A Hutchinson, and D S Robinson

The lipoprotein lipase activity of rat epididymal adipose tissue falls on starvation and increases on refeeding. Studies with fat cells isolated from this tissue have shown that increases in the activity of the enzyme occur under appropriate incubation conditions in vitro. The present study compares the responses of cells isolated from the adipose tissue of fed and 48-h starved rats. Fat cells from rats starved for 48 h display a lower initial lipoprotein lipase activity than cells from fed rats. When cells from rats in both nutritional states are incubated in a suitable medium at 25 degrees C, there is a progressive increase in the medium lipoprotein lipase activity. The absolute increase in the total activity of the incubation system during incubations of cells from 48-h starved rats is significantly less than during incubations of cells from fed rats. However, when expressed as a percentage of the initial cell activity, the rises in total activity are similar in the two nutritional states. Cycloheximide has no significant effect on the increase in activity of lipoprotein lipase that occurs with cells from 48-h starved rats. However, it does partially block the increase in activity seen with cells from fed rats and in a manner similar to that previously reported for cells from 24-h starved rats. The significance of the results is discussed in relation to previous studies with both intact adipose tissue and isolated fat cells.

UI	MeSH Term	Description	Entries
D008071	Lipoprotein Lipase	An enzyme of the hydrolase class that catalyzes the reaction of triacylglycerol and water to yield diacylglycerol and a fatty acid anion. The enzyme hydrolyzes triacylglycerols in chylomicrons, very-low-density lipoproteins, low-density lipoproteins, and diacylglycerols. It occurs on capillary endothelial surfaces, especially in mammary, muscle, and adipose tissue. Genetic deficiency of the enzyme causes familial hyperlipoproteinemia Type I. (Dorland, 27th ed) EC 3.1.1.34.	Heparin-Clearing Factor,Lipemia-Clearing Factor,Diacylglycerol Lipase,Diglyceride Lipase,Post-Heparin Lipase,Postheparin Lipase,Postheparin Lipoprotein Lipase,Factor, Heparin-Clearing,Factor, Lipemia-Clearing,Heparin Clearing Factor,Lipase, Diacylglycerol,Lipase, Diglyceride,Lipase, Lipoprotein,Lipase, Post-Heparin,Lipase, Postheparin,Lipase, Postheparin Lipoprotein,Lipemia Clearing Factor,Lipoprotein Lipase, Postheparin,Post Heparin Lipase
D008297	Male		Males
D004822	Epididymis	The convoluted cordlike structure attached to the posterior of the TESTIS. Epididymis consists of the head (caput), the body (corpus), and the tail (cauda). A network of ducts leaving the testis joins into a common epididymal tubule proper which provides the transport, storage, and maturation of SPERMATOZOA.
D000273	Adipose Tissue	Specialized connective tissue composed of fat cells (ADIPOCYTES). It is the site of stored FATS, usually in the form of TRIGLYCERIDES. In mammals, there are two types of adipose tissue, the WHITE FAT and the BROWN FAT. Their relative distributions vary in different species with most adipose tissue being white.	Fatty Tissue,Body Fat,Fat Pad,Fat Pads,Pad, Fat,Pads, Fat,Tissue, Adipose,Tissue, Fatty
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013217	Starvation	Lengthy and continuous deprivation of food. (Stedman, 25th ed)
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus