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Monoclonal antibodies were prepared to anti-HLA-DR cytolytic T lymphocytes (CTLs) and screened for inhibition of CTL-mediated killing. Binding of monoclonal antibodies to four types of molecules, LFA-1, LFA-2, LFA-3, and HLA-DR, inhibited killing, suggesting that these molecules participate in the CTL-target cell interaction. The antigens were characterized by immunoprecipitation, crosslinking, NaDodSO4/polyacrylamide gel electrophoresis, and immunofluorescence flow cytometry. The LFA-1 antigen contains alpha and beta polypeptide chains of Mr 177,000 and 95,000 that are noncovalently associated in an alpha 1 beta 1 structure. It is present on both B and T lymphocytes and marks subpopulations that differ in quantitative expression. Human LFA-1 appears to be the homologue of mouse LFA-1. Human LFA-2 is of Mr 49,000 with a minor component of Mr 36,000. It is expressed on CTL lines but not on a B-cell line and in peripheral blood preferentially on T lymphocytes. Human LFA-3 is of Mr 60,000 and is expressed on both B and T lymphocytes.
F Sanchez-Madrid, and
A M Krensky, and
C F Ware, and
E Robbins, and
J L Strominger, and
S J Burakoff, and
T A Springer
July 1981,
Proceedings of the National Academy of Sciences of the United States of America,
F Sanchez-Madrid, and
A M Krensky, and
C F Ware, and
E Robbins, and
J L Strominger, and
S J Burakoff, and
T A Springer
January 1982,
Immunological reviews,
F Sanchez-Madrid, and
A M Krensky, and
C F Ware, and
E Robbins, and
J L Strominger, and
S J Burakoff, and
T A Springer
August 1987,
Journal of immunology (Baltimore, Md. : 1950),
F Sanchez-Madrid, and
A M Krensky, and
C F Ware, and
E Robbins, and
J L Strominger, and
S J Burakoff, and
T A Springer
April 1988,
Biochemistry,
F Sanchez-Madrid, and
A M Krensky, and
C F Ware, and
E Robbins, and
J L Strominger, and
S J Burakoff, and
T A Springer
May 1984,
Journal of immunology (Baltimore, Md. : 1950),
F Sanchez-Madrid, and
A M Krensky, and
C F Ware, and
E Robbins, and
J L Strominger, and
S J Burakoff, and
T A Springer
January 1977,
Cellular immunology,
F Sanchez-Madrid, and
A M Krensky, and
C F Ware, and
E Robbins, and
J L Strominger, and
S J Burakoff, and
T A Springer
July 2009,
The Journal of biological chemistry,
F Sanchez-Madrid, and
A M Krensky, and
C F Ware, and
E Robbins, and
J L Strominger, and
S J Burakoff, and
T A Springer
September 1994,
European journal of immunology,
F Sanchez-Madrid, and
A M Krensky, and
C F Ware, and
E Robbins, and
J L Strominger, and
S J Burakoff, and
T A Springer
August 1981,
Journal of immunology (Baltimore, Md. : 1950),
F Sanchez-Madrid, and
A M Krensky, and
C F Ware, and
E Robbins, and
J L Strominger, and
S J Burakoff, and
T A Springer
November 1982,
Nature,
