BIOMAT Database Logo BIOMAT Database Logo

Technique for determining total bacterial virus counts in complex aqueous systems. 1980

D L Ewert, and M J Paynter

A direct method is described for measuring bacteriophage concentrations in complex aqueous systems. Conditions for sample clarification, phage recognition, and recovery were optimized. In contrast to the plaque assay, this procedure permits quantitation of total numbers of phages independent of bacterial host. Also, the modifications increase the sensitivity of the sedimentation assay, permitting detection of particles at a minimum concentration of 10(4) per ml. Maximal total phage concentrations in the aqueous phase of sewage and activated sludge mixed liquor were 1.3 x 10(6) and 4.3 x 10(7) per ml, respectively.

UI MeSH Term Description Entries
D008828 Microbiological Techniques Techniques used in microbiology. Microbiologic Technic,Microbiologic Technics,Microbiologic Technique,Microbiological Technics,Technic, Microbiologic,Technics, Microbiological,Technique, Microbiologic,Techniques, Microbiologic,Microbiologic Techniques,Microbiological Technic,Microbiological Technique,Technic, Microbiological,Technics, Microbiologic,Technique, Microbiological,Techniques, Microbiological
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002498 Centrifugation Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001435 Bacteriophages Viruses whose hosts are bacterial cells. Phages,Bacteriophage,Phage
D012417 Rumen The first stomach of ruminants. It lies on the left side of the body, occupying the whole of the left side of the abdomen and even stretching across the median plane of the body to the right side. It is capacious, divided into an upper and a lower sac, each of which has a blind sac at its posterior extremity. The rumen is lined by mucous membrane containing no digestive glands, but mucus-secreting glands are present in large numbers. Coarse, partially chewed food is stored and churned in the rumen until the animal finds circumstances convenient for rumination. When this occurs, little balls of food are regurgitated through the esophagus into the mouth, and are subjected to a second more thorough mastication, swallowed, and passed on into other parts of the compound stomach. (From Black's Veterinary Dictionary, 17th ed) Rumens
D012722 Sewage Refuse liquid or waste matter carried off by sewers. Sludge,Sludge Flocs

Related Publications

D L Ewert, and M J Paynter
Collaborative Evaluation of the Plate Loop Technique for Determining Viable Bacterial Counts in Raw Milk.
April 1980, Journal of food protection,
D L Ewert, and M J Paynter
A simplified technique for determining significant colony counts in urine.
October 1966, American journal of obstetrics and gynecology,
D L Ewert, and M J Paynter
Charts for determining the percentage variation of total leucocyte counts.
May 1946, American journal of clinical pathology,
D L Ewert, and M J Paynter
A Comparative Study of Standard Agars for Determining Bacterial Counts in Water.
April 1941, American journal of public health and the nation's health,
D L Ewert, and M J Paynter
[Titration technique for determining bacterial sensitivity to antibiotics].
January 1951, Annales de biologie clinique,
D L Ewert, and M J Paynter
Total counts of bacterial spores using counting slides.
September 1962, Journal of general microbiology,
D L Ewert, and M J Paynter
[Correlation between total aqueous protein concentrations and photon counts in rabbits].
November 1990, Nippon Ganka Gakkai zasshi,
D L Ewert, and M J Paynter
[Viable bacterial counts by agar-droplet technique (author's transl)].
August 1976, Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie,
D L Ewert, and M J Paynter
A contact-plate technique for determining bacterial contamination of fabrics.
August 1960, Lancet (London, England),
D L Ewert, and M J Paynter
Machine learning potentials for complex aqueous systems made simple.
September 2021, Proceedings of the National Academy of Sciences of the United States of America,
Copied contents to your clipboard!