A comparison of four commercial systems for the identification of nonfermentative gram-negative bacilli. 1980

N M Burdash, and E R Bannister, and J P Manos, and M E West

Four commercial systems for the identification of glucose nonfermentative, gram-negative bacilli and the conventional system used in our laboratory were compared. The API 20E, Roche Oxi/Ferm Tube, BBL Minitek, Flow N/F System, and our system were compared for accuracy, time needed for identification and number of isolates needing additional testing. A total of 241 different isolates were used. The API 20E identified 86.7% correctly; Oxi/FermTube, 83.0%; Minitek, 93.4%; N/F System, 90.5%; and our system, 90.0%. Within 24 hours after isolation, the API 20E identified 25.7%; Oxi/Ferm Tube and Minitek, 0%; N/F System 27.3%; and our system, 35.7%. After 48 hours, the API 20E identified 74.7%; Oxi/Ferm Tube, 35.7%; Minitek, 45.2%; N/F System, 83.0%; and our system, 94.6%. The number of isolates that required additional testing for identification by the API 20E was 119; Oxi/Ferm Tube, 161; Minitek, 88; N/F System, 43; and our system, 36. Ease of performance, technologist time, and level of identification required by individual laboratories are discussed.

UI MeSH Term Description Entries
D011549 Pseudomonas A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants. Chryseomonas,Pseudomona,Flavimonas
D005285 Fermentation Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID. Fermentations
D001431 Bacteriological Techniques Techniques used in studying bacteria. Bacteriologic Technic,Bacteriologic Technics,Bacteriologic Techniques,Bacteriological Technique,Technic, Bacteriological,Technics, Bacteriological,Technique, Bacteriological,Techniques, Bacteriological,Bacteriologic Technique,Bacteriological Technic,Bacteriological Technics,Technic, Bacteriologic,Technics, Bacteriologic,Technique, Bacteriologic,Techniques, Bacteriologic

Related Publications

N M Burdash, and E R Bannister, and J P Manos, and M E West
June 1976, Journal of clinical microbiology,
N M Burdash, and E R Bannister, and J P Manos, and M E West
May 1976, Human pathology,
N M Burdash, and E R Bannister, and J P Manos, and M E West
June 1970, The American journal of medicine,
N M Burdash, and E R Bannister, and J P Manos, and M E West
December 1987, Medicina clinica,
N M Burdash, and E R Bannister, and J P Manos, and M E West
December 2007, Journal of clinical microbiology,
N M Burdash, and E R Bannister, and J P Manos, and M E West
June 1978, Journal of clinical microbiology,
N M Burdash, and E R Bannister, and J P Manos, and M E West
April 1975, Antimicrobial agents and chemotherapy,
N M Burdash, and E R Bannister, and J P Manos, and M E West
August 1976, The Journal of infectious diseases,
N M Burdash, and E R Bannister, and J P Manos, and M E West
January 1982, Journal of clinical microbiology,
N M Burdash, and E R Bannister, and J P Manos, and M E West
March 1980, Taiwan yi xue hui za zhi. Journal of the Formosan Medical Association,
Copied contents to your clipboard!