Biomaterial Database

Evaluation of a multitest system for rapid identification of Salmonella and Shigella. 1980

W M Gooch

The ability of Micro-ID, a multitest system for rapid (four hour) identification of Enterobacteriaceae, to identify Salmonella and Shigella was evaluated. Micro-ID, API 20E and a battery of tubed media consisting of triple sugar-iron agar, Christensen's urea agar, and Moeller's lysine decarboxylase medium were used to study 516 lactose nonfermenting strains of Enterobacteriaceae isolated from 500 consecutive pediatric stool specimens. Fifty-six of the isolates were Salmonella, and 21 were Shigella. Micro-ID correctly identified all isolates of Shigella and all but one isolate of Salmonella, whereas the conventional screening media failed to detect seven isolates of Salmonella and two isolates of Shigella. The false-positive rates were 1% and 20% for Micro-ID and the conventional battery, respectively. Use of Micro-ID as a substitute for conventional screening media for lactose nonfermenting stool isolates provides reliable presumptive idenfication of Salmonella and Shigella within four hours.

UI	MeSH Term	Description	Entries
D008828	Microbiological Techniques	Techniques used in microbiology.	Microbiologic Technic,Microbiologic Technics,Microbiologic Technique,Microbiological Technics,Technic, Microbiologic,Technics, Microbiological,Technique, Microbiologic,Techniques, Microbiologic,Microbiologic Techniques,Microbiological Technic,Microbiological Technique,Technic, Microbiological,Technics, Microbiologic,Technique, Microbiological,Techniques, Microbiological
D004755	Enterobacteriaceae	A family of gram-negative, facultatively anaerobic, rod-shaped bacteria that do not form endospores. Its organisms are distributed worldwide with some being saprophytes and others being plant and animal parasites. Many species are of considerable economic importance due to their pathogenic effects on agriculture and livestock.	Coliform Bacilli,Enterobacteria,Ewingella,Leclercia,Paracolobactrum,Sodalis
D005069	Evaluation Studies as Topic	Works about studies that determine the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies.	Critique,Evaluation Indexes,Evaluation Methodology,Evaluation Report,Evaluation Research,Methodology, Evaluation,Pre-Post Tests,Qualitative Evaluation,Quantitative Evaluation,Theoretical Effectiveness,Use-Effectiveness,Critiques,Effectiveness, Theoretical,Evaluation Methodologies,Evaluation Reports,Evaluation, Qualitative,Evaluation, Quantitative,Evaluations, Qualitative,Evaluations, Quantitative,Indexes, Evaluation,Methodologies, Evaluation,Pre Post Tests,Pre-Post Test,Qualitative Evaluations,Quantitative Evaluations,Report, Evaluation,Reports, Evaluation,Research, Evaluation,Test, Pre-Post,Tests, Pre-Post,Use Effectiveness
D005189	False Positive Reactions	Positive test results in subjects who do not possess the attribute for which the test is conducted. The labeling of healthy persons as diseased when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed)	False Positive Reaction,Positive Reaction, False,Positive Reactions, False,Reaction, False Positive,Reactions, False Positive
D012475	Salmonella	A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that utilizes citrate as a sole carbon source. It is pathogenic for humans, causing enteric fevers, gastroenteritis, and bacteremia. Food poisoning is the most common clinical manifestation. Organisms within this genus are separated on the basis of antigenic characteristics, sugar fermentation patterns, and bacteriophage susceptibility.
D012760	Shigella	A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that ferments sugar without gas production. Its organisms are intestinal pathogens of man and other primates and cause bacillary dysentery (DYSENTERY, BACILLARY).

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