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As previously reported [Favilla, R. & Cavatorta, P. (1975) FEBS Lett. 50, 324-329], the enzyme horse liver alcohol dehydrogenase catalyzes a reaction between NAD+ and H2O2. The final isolated product was then called NADX because of its unknown structure. In this paper the results of spectroscopic investigations on this compound are described. They indicate that only the nicotinamide moiety of the original NAD+ molecule was modified by the action of hydrogen peroxide. From the 1H and 13C nuclear magnetic resonance spectra of NADX the following structure was deduced: adenosine(5')diphospho(5)-beta-D-ribose-NH-CH = C(CHO)-CONH2. This structure is consistent with both ultraviolet and reactivity measurements performed on NADX. A tentative mechanism for the whole peroxidatic reaction pathway leading to NADX is finally proposed.
A Mazzini, and
E Dradi, and
R Favilla, and
A Fava, and
P Cavatorta, and
M A Abdallah
February 1980,
European journal of biochemistry,
A Mazzini, and
E Dradi, and
R Favilla, and
A Fava, and
P Cavatorta, and
M A Abdallah
October 1988,
Biochimica et biophysica acta,
A Mazzini, and
E Dradi, and
R Favilla, and
A Fava, and
P Cavatorta, and
M A Abdallah
February 1980,
European journal of biochemistry,
A Mazzini, and
E Dradi, and
R Favilla, and
A Fava, and
P Cavatorta, and
M A Abdallah
August 1982,
Archives of biochemistry and biophysics,
A Mazzini, and
E Dradi, and
R Favilla, and
A Fava, and
P Cavatorta, and
M A Abdallah
January 1972,
Biochimica et biophysica acta,
A Mazzini, and
E Dradi, and
R Favilla, and
A Fava, and
P Cavatorta, and
M A Abdallah
January 1966,
Biochemical and biophysical research communications,
A Mazzini, and
E Dradi, and
R Favilla, and
A Fava, and
P Cavatorta, and
M A Abdallah
November 1975,
European journal of biochemistry,
A Mazzini, and
E Dradi, and
R Favilla, and
A Fava, and
P Cavatorta, and
M A Abdallah
December 1987,
European journal of biochemistry,
A Mazzini, and
E Dradi, and
R Favilla, and
A Fava, and
P Cavatorta, and
M A Abdallah
September 1982,
Biochemistry,
A Mazzini, and
E Dradi, and
R Favilla, and
A Fava, and
P Cavatorta, and
M A Abdallah
May 1988,
Biochemistry,
