Biomaterial Database

Effects of small concentrations of Mucor miehei protease on stability of sterile milk-based dietetic foods. 1980

R K Thunell, and C A Ernstrom, and G H Hartman

Four commercial sterile milk-based dietetic products were inoculated aseptically with small concentrations of a commericial milk clotting enzyme derived from Mucor miehei and incubated at 30 C for 32 wk. One of the products, Sustacal, exhibited no observable changes in body and texture during storage. Enzyme concentrations of 1 x 10(-4) chymosin units per milliliter or higher induced undesirable changes in the body of the other three products, Enfamil Ready-to-Use, Enfamil Concentrate, and Metrecal Shape. None of the products was affected visibly by enzyme concentrations of 1 x 10(-5) chymosin units per milliliter or less. In-can sterilization of Enfamil Ready-to-Use at 126.1 C for 4.5 min and Enfamil Concentrate at 126.7 C for 4.23 min completely destroyed all measurable enzyme activity at concentrations (up to 5.5 x 10(-2) chymosin units per milliliter) in these experiments. However, 1 to 3 h between formulation and sterilization allowed time for the enzyme at concentrations of 1 x 10(-2) chymosin units per milliliter or more to cause coagulation during heat sterilization.

UI	MeSH Term	Description	Entries
D007225	Infant Food	Food processed and manufactured for the nutritional health of children in their first year of life.	Food, Infant,Foods, Infant,Infant Foods
D009089	Mucor	A genus of zygomycetous fungi of the family Mucoraceae, order Mucorales. It is primarily saprophytic, but may cause MUCORMYCOSIS in man from spores germinating in the lungs.	Mucors
D010447	Peptide Hydrolases	Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.	Peptidase,Peptidases,Peptide Hydrolase,Protease,Proteases,Proteinase,Proteinases,Proteolytic Enzyme,Proteolytic Enzymes,Esteroproteases,Enzyme, Proteolytic,Hydrolase, Peptide
D003611	Dairy Products	Raw and processed or manufactured milk and milk-derived products. These are usually from cows (bovine) but are also from goats, sheep, reindeer, and water buffalo.	Dairy Product,Product, Dairy,Products, Dairy
D004032	Diet	Regular course of eating and drinking adopted by a person or animal.	Diets
D005502	Food	Substances taken in by the body to provide nourishment.	Foods
D013242	Sterilization	The destroying of all forms of life, especially microorganisms, by heat, chemical, or other means.