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In the presence of dUTP, net DNA synthesis in vitro is substantially reduced. Small DNA fragments that arise during in vitro DNA synthesis in the presence of dUTP are produced as a result of dUMP incorporation and subsequent post-replication excision repair process initiated by uracil-DNA-glycosylase. The size of the fragments is dependent upon the amount of dUMP incorporated, but unlike the normal 4S intermediates of DNA synthesis, these repair products are not precursors to high molecular weight DNA but are further degraded. The high levels of dUTPase as well as the presence of RNA primers on most nascent DNA pieces (Tseng and Goulian, 1977) suggest that repair of uracil-containing DNA does not contribute to the generation of the small, nascent DNA pieces found during DNA synthesis in this in vitro system.
R H Grafstrom, and
B Y Tseng, and
M Goulian
March 1979,
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R H Grafstrom, and
B Y Tseng, and
M Goulian
January 1979,
Cold Spring Harbor symposia on quantitative biology,
R H Grafstrom, and
B Y Tseng, and
M Goulian
June 1973,
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R H Grafstrom, and
B Y Tseng, and
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September 1962,
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R H Grafstrom, and
B Y Tseng, and
M Goulian
October 1984,
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R H Grafstrom, and
B Y Tseng, and
M Goulian
April 1975,
Biochemical and biophysical research communications,
R H Grafstrom, and
B Y Tseng, and
M Goulian
January 1969,
Biopolymers,
R H Grafstrom, and
B Y Tseng, and
M Goulian
March 1958,
Archives of biochemistry and biophysics,
R H Grafstrom, and
B Y Tseng, and
M Goulian
March 1990,
Mutation research,
R H Grafstrom, and
B Y Tseng, and
M Goulian
May 1978,
Proceedings of the National Academy of Sciences of the United States of America,
