Biomaterial Database

Methodological studies of monocyte yeast cell phagocytosis. 1980

B Svensson

Human mononuclear cells were separated in a Ficoll-Isopaque gradient and incubated in glass chambers in a medium of serum in Parker 199. At the end of the incubation time heat-killed, not pre-opsonized yeast cells (YC) were added for phagocytosis. PA was expressed as the median number of YC per monocyte in 100 monocytes. The results of testing various modifications of this method indicate that an appropriate procedure, allowing a sufficient span for measuring 'subnormal' phagocytosis, is incubation of the monocytes in 15 per cent serum for 120 minutes followed by a further incubation with 10(7) YC (in 7.5% serum) for 30 minutes. The sources of variation in the determination of PA of a standard population was studied by analysis of variance. Based on the results of this study, suitable ways to define and express 'normal' and 'low' phagocytic activity were proposed.

UI	MeSH Term	Description	Entries
D007158	Immunologic Techniques	Techniques used to demonstrate or measure an immune response, and to identify or measure antigens using antibodies.	Antibody Dissociation,Immunologic Technic,Immunologic Technics,Immunologic Technique,Immunological Technics,Immunological Techniques,Technic, Immunologic,Technics, Immunologic,Technique, Immunologic,Techniques, Immunologic,Antibody Dissociations,Dissociation, Antibody,Dissociations, Antibody,Immunological Technic,Immunological Technique,Technic, Immunological,Technics, Immunological,Technique, Immunological,Techniques, Immunological
D008297	Male		Males
D009000	Monocytes	Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.	Monocyte
D010587	Phagocytosis	The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).	Phagocytoses
D002448	Cell Adhesion	Adherence of cells to surfaces or to other cells.	Adhesion, Cell,Adhesions, Cell,Cell Adhesions
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D005260	Female		Females
D005898	Glass	Hard, amorphous, brittle, inorganic, usually transparent, polymerous silicate of basic oxides, usually potassium or sodium. It is used in the form of hard sheets, vessels, tubing, fibers, ceramics, beads, etc.
D006358	Hot Temperature	Presence of warmth or heat or a temperature notably higher than an accustomed norm.	Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man