Biomaterial Database

Enzymic micro-assay for blood glutamine. 1978

A Stahl, and A Frick, and M Imler, and J L Schlienger

Blood glutamine is hydrolyzed by glutaminase; the resulting ammonia is dialyzed out and measured by use of a continuous-flow colorimetric microassay. Concentrations in the range of 10 to 1300 micromol of glutamine per liter can be determined in a 40-microliter sample. With slight modifications, higher concentrations, up to 5 mmol/liter, can be measured. Assay conditions and the qualities of the microtechnique were made optimal. Usual values obtained with this method for arterial and venous blood in man are, respectively, 443 +/- 140 and 466 +/- 142 micromol/liter (mean +/- 2 SD). Corresponding values for rats are 320 +/- 92 and 340 +/- 108 micromol/liter. Fasting causes a decreased blood glutamine.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D008722	Methods	A series of steps taken in order to conduct research.	Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D008832	Microchemistry	The development and use of techniques and equipment to study or perform chemical reactions, with small quantities of materials, frequently less than a milligram or a milliliter.
D008875	Middle Aged	An adult aged 45 - 64 years.	Middle Age
D010424	Pentobarbital	A short-acting barbiturate that is effective as a sedative and hypnotic (but not as an anti-anxiety) agent and is usually given orally. It is prescribed more frequently for sleep induction than for sedation but, like similar agents, may lose its effectiveness by the second week of continued administration. (From AMA Drug Evaluations Annual, 1994, p236)	Mebubarbital,Mebumal,Diabutal,Etaminal,Ethaminal,Nembutal,Pentobarbital Sodium,Pentobarbital, Monosodium Salt,Pentobarbitone,Sagatal,Monosodium Salt Pentobarbital
D012016	Reference Values	The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.	Normal Range,Normal Values,Reference Ranges,Normal Ranges,Normal Value,Range, Normal,Range, Reference,Ranges, Normal,Ranges, Reference,Reference Range,Reference Value,Value, Normal,Value, Reference,Values, Normal,Values, Reference
D005019	Ethyl Ethers	Organic compounds having ethyl groups bound to an oxygen atom.	Ethoxy Compounds,Compounds, Ethoxy,Ethers, Ethyl
D005215	Fasting	Abstaining from FOOD.	Hunger Strike,Hunger Strikes,Strike, Hunger,Strikes, Hunger
D005260	Female		Females
D005972	Glutaminase		Phosphate-Activated Glutaminase,Glutaminase, Phosphate-Activated,Phosphate Activated Glutaminase