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By S1 nuclease mapping we have located the intervening sequence in the large ribosomal RNA gene of Saccharomyces cerevisiae omega+ strains 570 bp from the 3' end of the rRNA gene. No intervening sequence was detected at this position in S. carlsbergensis, but the sequences of the mature 21S rRNAs of these two strains appear to be identical in this region. By comparing the DNA sequence of the region of the intervening sequence in an omega+ strain with the corresponding sequence in S. carlsbergensis, we have determined the splice points of the 21S rRNA gene. These sequences show no homology with splice points in nuclear and viral genes or with the splice points in the chloroplast 23S rRNA gene of Chlamydomonas. The external borders of the splice points have a complementary sequence in the intervening sequence. The largest transcript hybridizing with the probe of the intervening sequence has a size corresponding to that expected for an rRNA precursor still containing the intervening sequence; the smallest transcript corresponds in size to the intervening sequence itself.
J L Bos, and
K A Osinga, and
G Van der Horst, and
N B Hecht, and
H F Tabak, and
G J Van Ommen, and
P Borst
January 1982,
Molecular & general genetics : MGG,
J L Bos, and
K A Osinga, and
G Van der Horst, and
N B Hecht, and
H F Tabak, and
G J Van Ommen, and
P Borst
December 1979,
Current genetics,
J L Bos, and
K A Osinga, and
G Van der Horst, and
N B Hecht, and
H F Tabak, and
G J Van Ommen, and
P Borst
January 1979,
Molecular & general genetics : MGG,
J L Bos, and
K A Osinga, and
G Van der Horst, and
N B Hecht, and
H F Tabak, and
G J Van Ommen, and
P Borst
December 1980,
Comptes rendus des seances de l'Academie des sciences. Serie D, Sciences naturelles,
J L Bos, and
K A Osinga, and
G Van der Horst, and
N B Hecht, and
H F Tabak, and
G J Van Ommen, and
P Borst
July 1983,
Proceedings of the National Academy of Sciences of the United States of America,
J L Bos, and
K A Osinga, and
G Van der Horst, and
N B Hecht, and
H F Tabak, and
G J Van Ommen, and
P Borst
March 1980,
Proceedings of the National Academy of Sciences of the United States of America,
J L Bos, and
K A Osinga, and
G Van der Horst, and
N B Hecht, and
H F Tabak, and
G J Van Ommen, and
P Borst
January 1979,
Molecular & general genetics : MGG,
J L Bos, and
K A Osinga, and
G Van der Horst, and
N B Hecht, and
H F Tabak, and
G J Van Ommen, and
P Borst
January 1979,
Molecular & general genetics : MGG,
J L Bos, and
K A Osinga, and
G Van der Horst, and
N B Hecht, and
H F Tabak, and
G J Van Ommen, and
P Borst
March 1979,
Cell,
J L Bos, and
K A Osinga, and
G Van der Horst, and
N B Hecht, and
H F Tabak, and
G J Van Ommen, and
P Borst
January 1981,
Molecular & general genetics : MGG,
