Biomaterial Database

[A study of the single radial haemolysis optimization for complement haemolytic activity assay (author's transl)]. 1980

E Bourret, and L Bardet

The experimental conditions for single radial haemolysis are analysed in order to optimize the haemolytic plate method for the assay of human total complement. The amount of haemolytic antibody used for sensitization of red cells, the concentration of sensitized sheep erythrocytes, the thickness of agarose layer, and the diameters of wells all exercise an influence on the area of haemolysis. The effects of these parameters are investigated with a view to obtaining the clearest reading and greatest sensitivity of the haemolytic activity plate method, and at the same time verifying the validity of the assay and the precision of the human serum haemolytic titres. This work reports the optimal experimental requirements with regard to the factors studied for minimizing the sources of error in complement haemolytic plate assay.

UI	MeSH Term	Description	Entries
D003165	Complement System Proteins	Serum glycoproteins participating in the host defense mechanism of COMPLEMENT ACTIVATION that creates the COMPLEMENT MEMBRANE ATTACK COMPLEX. Included are glycoproteins in the various pathways of complement activation (CLASSICAL COMPLEMENT PATHWAY; ALTERNATIVE COMPLEMENT PATHWAY; and LECTIN COMPLEMENT PATHWAY).	Complement Proteins,Complement,Complement Protein,Hemolytic Complement,Complement, Hemolytic,Protein, Complement,Proteins, Complement,Proteins, Complement System
D006462	Hemolytic Plaque Technique	A method to identify and enumerate cells that are synthesizing ANTIBODIES against ANTIGENS or HAPTENS conjugated to sheep RED BLOOD CELLS. The sheep red blood cells surrounding cells secreting antibody are lysed by added COMPLEMENT producing a clear zone of HEMOLYSIS. (From Illustrated Dictionary of Immunology, 3rd ed)	Jerne's Plaque Technique,Hemolytic Plaque Technic,Jerne's Plaque Technic,Hemolytic Plaque Technics,Hemolytic Plaque Techniques,Jerne Plaque Technic,Jerne Plaque Technique,Jernes Plaque Technic,Jernes Plaque Technique,Plaque Technic, Hemolytic,Plaque Technic, Jerne's,Plaque Technics, Hemolytic,Plaque Technique, Hemolytic,Plaque Technique, Jerne's,Plaque Techniques, Hemolytic,Technic, Hemolytic Plaque,Technic, Jerne's Plaque,Technics, Hemolytic Plaque,Technique, Hemolytic Plaque,Technique, Jerne's Plaque,Techniques, Hemolytic Plaque
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

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