Biomaterial Database

Occurrence of N-acetylglucosamine-1-phosphate in proteinase I from Dictyostelium discoideum. 1980

G L Gustafson, and L A Milner

A previous study (Gustafson, G.L., and Thon, L. A. (1979) Biochem. Biophys. Res. Commun. 86, 667-673) demonstrated that Proteinase I from the cellular slime mold, Dictyostelium discoideum, was conjugated with phosphoryl moieties. This report describes a characterization of the covalent structure of these moieties. Essentially all of the phosphate associated with purified enzyme was released as a sugar-phosphate during mild alkaline hydrolysis. The sugar-phosphate was isolated from alkaline hydrolysates of Proteinase I by Sephadex G-25 chromatography and identified as the alpha-anomer of N-acetylglucosamine-1-phosphate. In separate experiments, involving acid hydrolysis of Proteinase I, it was shown that enzyme-phosphate could also be isolated as O-phosphorylserine. Based on the recovery of O-phosphorylserine from acid hydrolysates, it was concluded that the majority of the N-acetylglucosamine-1-phosphate residues in the proteinase were esterified to peptidyl serines through phosphoester bonds.

UI	MeSH Term	Description	Entries
D010450	Endopeptidases	A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS.	Endopeptidase,Peptide Peptidohydrolases
D010750	Phosphoproteins		Phosphoprotein
D010768	Phosphoserine	The phosphoric acid ester of serine.	Serine Phosphate,Phosphorylserine,Seryl Phosphate,Phosphate, Serine,Phosphate, Seryl
D011485	Protein Binding	The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.	Plasma Protein Binding Capacity,Binding, Protein
D004023	Dictyostelium	A genus of protozoa, formerly also considered a fungus. Its natural habitat is decaying forest leaves, where it feeds on bacteria. D. discoideum is the best-known species and is widely used in biomedical research.	Dictyostelium discoideum,Dictyostelium discoideums,Dictyosteliums,discoideum, Dictyostelium
D005944	Glucosamine		2-Amino-2-Deoxyglucose,Dona,Dona S,Glucosamine Sulfate,Hespercorbin,Xicil,2 Amino 2 Deoxyglucose,Sulfate, Glucosamine
D005958	Glucosephosphates
D000117	Acetylglucosamine	The N-acetyl derivative of glucosamine.	Acetyl Glucosamine,N-Acetyl Glucosamine,N-Acetyl-beta-D-Glucosamine,N-Acetylglucosamine,beta-N-Acetylglucosamine,2-Acetamido-2-Deoxy-D-Glucose,2-Acetamido-2-Deoxyglucose,N-Acetyl-D-Glucosamine,2 Acetamido 2 Deoxy D Glucose,2 Acetamido 2 Deoxyglucose,Glucosamine, Acetyl,Glucosamine, N-Acetyl,N Acetyl D Glucosamine,N Acetyl Glucosamine,N Acetyl beta D Glucosamine,N Acetylglucosamine,beta N Acetylglucosamine
D012697	Serine Endopeptidases	Any member of the group of ENDOPEPTIDASES containing at the active site a serine residue involved in catalysis.	Serine Endopeptidase,Endopeptidase, Serine,Endopeptidases, Serine