Biomaterial Database

Peptones and calf serum as a replacement for human serum in the cultivation of Plasmodium falciparum. 1980

T Ifediba, and J P Vanderberg

Attempts were made to find an inexpensive, readily available substitute for human serum requirement for the continuous culture of erythrocytic stages of Plasmodium falciparum. We found that Neopeptone or Proteose-Peptone No. 3 added together with calf serum gave parasite growth rates comparable to, or surpassing, those obtained with human serum. However, first it was necessary to adapt the parasites by a gradual, stepwise reduction in the amount of human serum used, and a concomitant, stepwise increase in the substitutes added.

UI	MeSH Term	Description	Entries
D010274	Parasitology	The study of parasites and PARASITIC DISEASES.	Medical Parasitology,Parasitology, Medical
D010461	Peptones	Derived proteins or mixtures of cleavage products produced by the partial hydrolysis of a native protein either by an acid or by an enzyme. Peptones are readily soluble in water, and are not precipitable by heat, by alkalis, or by saturation with ammonium sulfate. (Dorland, 28th ed)	Peptone
D010963	Plasmodium falciparum	A species of protozoa that is the causal agent of falciparum malaria (MALARIA, FALCIPARUM). It is most prevalent in the tropics and subtropics.	Plasmodium falciparums,falciparums, Plasmodium
D001769	Blood	The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D004912	Erythrocytes	Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.	Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000222	Adaptation, Physiological	The non-genetic biological changes of an organism in response to challenges in its ENVIRONMENT.	Adaptation, Physiologic,Adaptations, Physiologic,Adaptations, Physiological,Adaptive Plasticity,Phenotypic Plasticity,Physiological Adaptation,Physiologic Adaptation,Physiologic Adaptations,Physiological Adaptations,Plasticity, Adaptive,Plasticity, Phenotypic
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia

Related Publications

T Ifediba, and J P Vanderberg

Continuous in vitro cultivation of Plasmodium falciparum: replacement of human serum by calf serum and proteose peptone.

January 1981, The Indian journal of medical research,

T Ifediba, and J P Vanderberg

Cultivation of Plasmodium falciparum using animal serum (horse, calf and bovine) as human serum substitute.

November 1986, Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology,

T Ifediba, and J P Vanderberg

Sheep serum as a replacement to human serum for the maintenance of in vitro cultures of Plasmodium falciparum.

March 1981, The Journal of communicable diseases,

T Ifediba, and J P Vanderberg

Fatty acid-free bovine albumin as plasma replacement for in vitro cultivation of Plasmodium falciparum.

June 1977, The Journal of parasitology,

T Ifediba, and J P Vanderberg

Cultivation of Plasmodium falciparum parasites in a serum-free medium.

September 1993, The American journal of tropical medicine and hygiene,

T Ifediba, and J P Vanderberg

Serum-free cultivation of several Plasmodium falciparum strains.

January 1994, Parasitology research,