Biomaterial Database

Ribosomal precursor RNA metabolism and cell division in the yeast Saccharomyces cerevisiae. 1980

G C Johnston, and R A Singer

When shifted from 23 degrees C to 36 degrees C, cells of a non-temperature-sensitive strain of yeast arrest transiently in G1 before continuation of the cell division cycle. When shifted to 36 degrees C, cells harboring a temperature-sensitive rna mutation behave similarly. Others have shown that temperature shift transiently decreases the rates of production and processing of ribosomal precursor RNA (rpreRNA). Production of rpreRNA is soon restored to normal levels in these strains, but normal processing of these repreRNA transcripts is restored only in non-temperature-sensitive strains. Therefore these experiments serve to eliminate from cell cycle considerations the involvement of processing of rpreRNA, while maintaining the established correlation between cell cycle behavior and rpreRNA production.

UI	MeSH Term	Description	Entries
D009694	Nucleic Acid Precursors	Use for nucleic acid precursors in general or for which there is no specific heading.	Acid Precursors, Nucleic,Precursors, Nucleic Acid
D010641	Phenotype	The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.	Phenotypes
D002455	Cell Division	The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.	M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D006358	Hot Temperature	Presence of warmth or heat or a temperature notably higher than an accustomed norm.	Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D012331	RNA, Fungal	Ribonucleic acid in fungi having regulatory and catalytic roles as well as involvement in protein synthesis.	Fungal RNA
D012335	RNA, Ribosomal	The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)	Ribosomal RNA,15S RNA,RNA, 15S
D012441	Saccharomyces cerevisiae	A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.	Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker