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Changes in extracellular pH affected insulin output from pancreatic islets stimulated with either glucose or alpha-ketoisocaproate. The extracellular pH at which the highest secretory response occurred was not identical in all cases, being shifted to alkaline values as the concentration of the nutrient secretagogue was increased. The output of insulin correlated with the nutrient-induced increment in 45Ca net uptake, but not with the rate of nutrient oxidation. By reference to changes in intracellular pH observed in islets exposed to either acidic media or increasing concentrations of alpha-ketoisocaproate, and taking into account a titration curve for the buffering capacity of islet homogenates, the optimal intracellular pH for insulin release was found to approximate a value of 0.09 pH unit below basal pH. It is postulated that, in the process of nutrient-induced insulin release, modest changes in intracellular pH participate in the multifactorial coupling between metabolic, ionic and secretory events.
J C Hutton, and
A Sener, and
A Herchuelz, and
I Valverde, and
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August 1982,
Endocrinology,
J C Hutton, and
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I Valverde, and
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W J Malaisse
January 1983,
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,
J C Hutton, and
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W J Malaisse
January 1991,
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W J Malaisse
July 1983,
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J C Hutton, and
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A C Boschero, and
W J Malaisse
January 1972,
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J C Hutton, and
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I Valverde, and
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May 1977,
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August 1979,
Archives of biochemistry and biophysics,
J C Hutton, and
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I Valverde, and
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W J Malaisse
September 1974,
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J C Hutton, and
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I Valverde, and
A C Boschero, and
W J Malaisse
March 1980,
Endocrinology,
J C Hutton, and
A Sener, and
A Herchuelz, and
I Valverde, and
A C Boschero, and
W J Malaisse
November 1973,
Endocrinology,
