Biomaterial Database

Immunofluorescent studies in filariasis: antibody levels in jirds (Meriones unguiculatus) infected with Brugia malayi. 1980

M Singh, and G J Kane, and C Burren, and L M Mackinlay

The indirect fluorescent antibody technique has been applied to detect antibody levels in jirds (Meriones unguiculatus) infected with Brugia malayi. Sonicated antigens were prepared from microfilariae and adult worms. Sonicated microfilariae were found to be satisfactory for this purpose. Cyanogen bromide-activated sepharose coated with soluble antigens prepared from microfilariae and adult worms was also used to detect antibodies to Brugia infections. The present observations show that these techniques can be usefully applied for detention of filarial infections. Antibody titres in infected jirds generally ranged from 1:16-1:256 and were not affected by treatment with diethylcarbamazine.

UI	MeSH Term	Description	Entries
D002009	Brugia	A filarial worm of Southeast Asia, producing filariasis and elephantiasis in various mammals including man. It was formerly included in the genus WUCHERERIA.	Brugias
D004049	Diethylcarbamazine	An anthelmintic used primarily as the citrate in the treatment of filariasis, particularly infestations with Wucheria bancrofti or Loa loa.	Carbamazine,Diethylcarbamazine Citrate,Diethylcarbamazine Citrate (1:1),Diethylcarbamazine Citrate (1:2),Diethylcarbamazine L-Tartrate (1:1),Diethylcarbamazine Maleate,Diethylcarbamazine Monohydrochloride,Diethylcarbamazine Phosphate (1:1),Hetrazan,Loxuran,Notezine,Citrate, Diethylcarbamazine,Maleate, Diethylcarbamazine,Monohydrochloride, Diethylcarbamazine
D005368	Filariasis	Infections with nematodes of the superfamily FILARIOIDEA. The presence of living worms in the body is mainly asymptomatic but the death of adult worms leads to granulomatous inflammation and permanent fibrosis. Organisms of the genus Elaeophora infect wild elk and domestic sheep causing ischemic necrosis of the brain, blindness, and dermatosis of the face.	Elaeophoriasis,Filarioidea Infections,Infections, Filarioidea,Elaeophoriases,Filariases,Filarioidea Infection,Infection, Filarioidea
D005370	Filarioidea	A superfamily of nematodes of the order SPIRURIDA. Members possess a filiform body and a mouth surrounded by papillae.	Litomosoides,Filarioideas,Litomosoide
D005455	Fluorescent Antibody Technique	Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.	Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000906	Antibodies	Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
D012377	Rodentia	A mammalian order which consists of 29 families and many genera.	Beavers,Capybaras,Castor Beaver,Dipodidae,Hydrochaeris,Jerboas,Rodents,Beaver,Capybara,Hydrochaeri,Jerboa,Rodent,Rodentias
D012967	Sodium Dodecyl Sulfate	An anionic surfactant, usually a mixture of sodium alkyl sulfates, mainly the lauryl; lowers surface tension of aqueous solutions; used as fat emulsifier, wetting agent, detergent in cosmetics, pharmaceuticals and toothpastes; also as research tool in protein biochemistry.	Sodium Lauryl Sulfate,Irium,Dodecyl Sulfate, Sodium,Lauryl Sulfate, Sodium,Sulfate, Sodium Dodecyl,Sulfate, Sodium Lauryl