Biomaterial Database

[Organization of mammalian genome replication: data on the high rate of DNA replication in replicons of structural heterochromatin]. 1980

N M Khaitova, and G S Il'ina, and N A Liapunova

By the autoradiography, the increase of the mean rate of replication fork movement in DNA was shown for human skin fibroblasts during S-period induced by reseeding the stationary culture; the portion of high-rate replicons growing parallel to the increase in numbers of cells, in which the constitutive heterochromatin was replicating. Furthermore, the heterogeneity of replicons was investigated in regard to the rate of DNA chain growth in asynchronous cell populations of human and three rodent species (Microtus agrestis, Tscherskia triton, Cricetulus griseus). A positive correlation was found between the portion of replicons, with a relatively high rate of replication (more than 60 micron/h), and the relative amounts of C-heterochromatin in genomes of these species. On the basis of these studies it has been assumed, that the replicons with the high rate of DNA chain growth belong to the constitutive heterochromatin.

UI	MeSH Term	Description	Entries
D007399	Interphase	The interval between two successive CELL DIVISIONS during which the CHROMOSOMES are not individually distinguishable. It is composed of the G phases (G1 PHASE; G0 PHASE; G2 PHASE) and S PHASE (when DNA replication occurs).	Interphases
D008214	Lymphocytes	White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.	Lymphoid Cells,Cell, Lymphoid,Cells, Lymphoid,Lymphocyte,Lymphoid Cell
D012093	Replicon	Any DNA sequence capable of independent replication or a molecule that possesses a REPLICATION ORIGIN and which is therefore potentially capable of being replicated in a suitable cell. (Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)	Replication Unit,Replication Units,Replicons,Unit, Replication,Units, Replication
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D003411	Arvicolinae	A subfamily of MURIDAE found nearly world-wide and consisting of about 20 genera. Voles, lemmings, and muskrats are members.	Clethrionomys,Cricetidae,Dicrostonyx,Lemmings,Lemmus,Mice, Red-Backed,Microtinae,Microtus,Muskrats,Ondatra,Voles,Arvicolines,Microtines,Mouse, Red-Backed,Myodes,Ondatra zibethicus,Arvicoline,Lemming,Mice, Red Backed,Microtine,Mouse, Red Backed,Muskrat,Red-Backed Mice,Red-Backed Mouse,Vole
D004261	DNA Replication	The process by which a DNA molecule is duplicated.	Autonomous Replication,Replication, Autonomous,Autonomous Replications,DNA Replications,Replication, DNA,Replications, Autonomous,Replications, DNA
D005347	Fibroblasts	Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.	Fibroblast
D006224	Cricetinae	A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.	Cricetus,Hamsters,Hamster
D006570	Heterochromatin	The portion of chromosome material that remains condensed and is transcriptionally inactive during INTERPHASE.	Heterochromatins
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man