Biomaterial Database

Pattern of meso-dl-2,6-diaminopimelic acid incorporation during the division cycle of Escherichia coli. 1980

R W Verwer, and N Nanninga

The topography of meso-DL-2,6-diaminopimelic acid incorportion into the cell envelope of Escherichia coli W7 (doubling time, tau, = 70 min) has been studied by autoradiography. To follow the incorporation pattern during the division cycle, cells have been classified according to length and the silver grain distributions have been determined in the two cell halves. In particular, the question of equivalence (with respect to the grain distributions) of the two cell halves has been investigated statistically. The grain localizations have been determined separately for the left cell halves (highest number of grains) and right cell halves. The highest probability of finding grains was in the central area for cells of all length classes. In the longest cells (dividing or nondividing) incorporation occurred in the future septal regions of the prospective daughter cells. Autoradiography of tritiated thymidine-labeled cells indicated the presence of an atypical deoxyribonucleic acid replication cycle (at tau = 70 min). Initiation of deoxyribonucleic acid replication occurred during the latter part of the division cycle, and its termination occurred in the next cycle.

UI	MeSH Term	Description	Entries
D008433	Mathematics	The deductive study of shape, quantity, and dependence. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Mathematic
D002455	Cell Division	The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.	M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D002462	Cell Membrane	The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.	Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D003960	Diaminopimelic Acid	A diamino derivative of heptanedioic acid with amino groups at C-2 and C-6 and the general formula (COOH)CH(NH2)CH2CH2CH2CH(NH2)(COOH).	2,6-Diaminopimelic Acid,2,6 Diaminopimelic Acid,Acid, 2,6-Diaminopimelic,Acid, Diaminopimelic
D004269	DNA, Bacterial	Deoxyribonucleic acid that makes up the genetic material of bacteria.	Bacterial DNA
D004926	Escherichia coli	A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.	Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D000599	Amino Acids, Diamino		Amino Acids, Dibasic,Diamino Amino Acids,Dibasic Amino Acids,Acids, Diamino Amino,Acids, Dibasic Amino