BIOMAT Database Logo BIOMAT Database Logo

Role of the sigma subunit of Escherichia coli RNA polymerase in initiation. II. Release of sigma from ternary complexes. 1980

U M Hansen, and W R McClure

The sigma subunit of Escherichia coli RNA polymerase was released from a transcribing polymerase-poly[d(A-T)]-poly[d(A-T)]-RNA complex when the nascent RNA reached a length of either eight or nine nucleotides. The dissociated sigma was separated from other reaction components by gel filtration, and was assayed using an activity assay previously described (Hansen, U. M., and McClure, W. R. (1979) J. Biol. Chem. 254, 5713-5717). The extent of RNA synthesis was limited by incorporation of 3'-dATP into the RNA chains; a series of distributions of product lengths was achieved by varying the concentration of 3'-dATP. A correlation of the number of moles of dissociated sigma versus the number of moles of RNA products of varying lengths allowed the determination of the point of release of the sigma subunit. Models to explain the cause of sigma release are discussed.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D011067 Poly dA-dT Polydeoxyribonucleotides made up of deoxyadenine nucleotides and thymine nucleotides. Present in DNA preparations isolated from crab species. Synthetic preparations have been used extensively in the study of DNA. Poly(Deoxyadenylate-Thymidylate),Polydeoxyadenine Nucleotides-Polythymine Nucleotides,Poly dA dT,Poly(dA-dT),d(A(5)T(5))2,Nucleotides, Polydeoxyadenine Nucleotides-Polythymine,Nucleotides-Polythymine Nucleotides, Polydeoxyadenine,Polydeoxyadenine Nucleotides Polythymine Nucleotides,dA dT, Poly,dA-dT, Poly,dT, Poly dA
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D012321 DNA-Directed RNA Polymerases Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992). DNA-Dependent RNA Polymerases,RNA Polymerases,Transcriptases,DNA-Directed RNA Polymerase,RNA Polymerase,Transcriptase,DNA Dependent RNA Polymerases,DNA Directed RNA Polymerase,DNA Directed RNA Polymerases,Polymerase, DNA-Directed RNA,Polymerase, RNA,Polymerases, DNA-Dependent RNA,Polymerases, DNA-Directed RNA,Polymerases, RNA,RNA Polymerase, DNA-Directed,RNA Polymerases, DNA-Dependent,RNA Polymerases, DNA-Directed
D013698 Templates, Genetic Macromolecular molds for the synthesis of complementary macromolecules, as in DNA REPLICATION; GENETIC TRANSCRIPTION of DNA to RNA, and GENETIC TRANSLATION of RNA into POLYPEPTIDES. Genetic Template,Genetic Templates,Template, Genetic
D014158 Transcription, Genetic The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION. Genetic Transcription
D046911 Macromolecular Substances Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure. Macromolecular Complexes,Macromolecular Compounds,Macromolecular Compounds and Complexes,Complexes, Macromolecular,Compounds, Macromolecular,Substances, Macromolecular

Related Publications

U M Hansen, and W R McClure
Release of the sigma subunit from Escherichia coli RNA polymerase transcription complexes is dependent on the promoter sequence.
September 1989, Biochemistry,
U M Hansen, and W R McClure
Role of the RNA polymerase sigma subunit in transcription initiation.
November 2002, Research in microbiology,
U M Hansen, and W R McClure
A gene from Escherichia coli affecting the sigma subunit of RNA polymerase.
May 1977, Proceedings of the National Academy of Sciences of the United States of America,
U M Hansen, and W R McClure
RNA chain initiation by Escherichia coli RNA polymerase. Structural transitions of the enzyme in early ternary complexes.
September 1989, Biochemistry,
U M Hansen, and W R McClure
Role of the sigma 70 subunit of Escherichia coli RNA polymerase in transcription activation.
January 1994, Journal of molecular biology,
U M Hansen, and W R McClure
The sigma subunit of Escherichia coli RNA polymerase senses promoter spacing.
August 1996, Proceedings of the National Academy of Sciences of the United States of America,
U M Hansen, and W R McClure
Domain organization of the Escherichia coli RNA polymerase sigma 70 subunit.
November 1996, Journal of molecular biology,
U M Hansen, and W R McClure
Transcription initiation by Escherichia coli RNA polymerase at the gene II promoter of M13 phage: stability of ternary complex, direct photocrosslinking to nascent RNA, and retention of sigma subunit.
November 1987, Biochimica et biophysica acta,
U M Hansen, and W R McClure
Overexpression and purification of the sigma subunit of Escherichia coli RNA polymerase.
December 1983, Gene,
U M Hansen, and W R McClure
Size of the unwound region of DNA in Escherichia coli RNA polymerase and calf thymus RNA polymerase II ternary complexes.
January 1983, Cold Spring Harbor symposia on quantitative biology,
Copied contents to your clipboard!