-transparent.png){kind=logo}
In isolated rat pancreatic islets, glucose (5.6, 11.1, and 16.7 mM) significantly increased reduced glutathione (GSH) and decreased oxidized glutathione (GSSG) levels in a dose-related manner. This was paralleled by a concomitant increase of NADPH and a decrease of NADP. The change of the GSH level occurred as quickly as one minute after addition of glucose. Exogenous insulin (200, 400, and 800 microU/ml) significantly decreased islet GSH levels in the presence of 5.6 and 16.7 mM glucose and significantly inhibited the insulin-releasing effect of the thiol reagent parachloromercuribenzoate (p-CMB) and tolbutamide. These data, together with earlier observations, suggest that GSH levels in pancreatic islets are increased by glucose and decreased by exogenous insulin via their effects on the pentose phosphate shunt and NADPH. Our results are compatible with the hypothesis that glucose and exogenous insulin, by modifying the redox state of the NADPH/NADP and GSH/GSSG systems, modulate the sensitivity of the beta-cell to the insulin-triggering actions of glucose, p-CMB, and tolbutamide.
H P Ammon, and
A Grimm, and
S Lutz, and
D Wagner-Teschner, and
M Händel, and
I Hagenloh
August 1979,
Diabetes,
H P Ammon, and
A Grimm, and
S Lutz, and
D Wagner-Teschner, and
M Händel, and
I Hagenloh
January 1993,
Advances in experimental medicine and biology,
H P Ammon, and
A Grimm, and
S Lutz, and
D Wagner-Teschner, and
M Händel, and
I Hagenloh
December 1981,
Diabete & metabolisme,
H P Ammon, and
A Grimm, and
S Lutz, and
D Wagner-Teschner, and
M Händel, and
I Hagenloh
January 1978,
Endocrinology,
H P Ammon, and
A Grimm, and
S Lutz, and
D Wagner-Teschner, and
M Händel, and
I Hagenloh
February 1973,
The Biochemical journal,
H P Ammon, and
A Grimm, and
S Lutz, and
D Wagner-Teschner, and
M Händel, and
I Hagenloh
January 1977,
Biochemical Society transactions,
H P Ammon, and
A Grimm, and
S Lutz, and
D Wagner-Teschner, and
M Händel, and
I Hagenloh
April 2007,
Biological & pharmaceutical bulletin,
H P Ammon, and
A Grimm, and
S Lutz, and
D Wagner-Teschner, and
M Händel, and
I Hagenloh
July 1990,
The American journal of physiology,
H P Ammon, and
A Grimm, and
S Lutz, and
D Wagner-Teschner, and
M Händel, and
I Hagenloh
January 1981,
The American journal of physiology,
H P Ammon, and
A Grimm, and
S Lutz, and
D Wagner-Teschner, and
M Händel, and
I Hagenloh
January 2021,
Nature communications,
