Biomaterial Database

Utilization of D-phenylglycyl-glycine in Escherichia coli. 1980

D Pfeifer, and J Kelley, and R Plapp

Escherichia coli K 12 is able to utilize the dipeptide D-phenylglycyl-glycine as a source of glycine. Growth experiments with a glycine auxotrophic mutant show that utilization of there dipeptide is competitively inhibited by D-alanine at a Ki of 4 x 10(-4) M. In contrast, L-alanyl-L-alanine which is transported via the system specific for dipeptides does not interfere with the utilization of D-phenylglycyl-glycine. This indicates that the dipeptide is hydrolyzed prior to uptake, and D-alanine therefore competes with the uptake of glycine via the transport system common for both amino acids. This was confirmed by examining the growth response of various transport mutants. A mutant deficient in the transport of oligo- and dipeptides grows as well as the wild type on D-phenylglycyl-glycine, whereas the growth of mutants deficient in the transport of glycine is severely impaired or prevented with this dipeptide. It is therefore demonstrated that D-phenylglycyl-glycine is hydrolyzed prior to uptake. This is a mechanism of peptide utilization in E. coli K 12 which is distinct from that described so far for other dipeptides.

UI	MeSH Term	Description	Entries
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D004151	Dipeptides	Peptides composed of two amino acid units.	Dipeptide
D004926	Escherichia coli	A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.	Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D006033	Glycylglycine	The simplest of all peptides. It functions as a gamma-glutamyl acceptor.	Diglycine,Glycyl-Glycine,Glycylglycine Hydrochloride,Glycylglycine Monohydrochloride,N-Glycylglycine,Glycyl Glycine,Hydrochloride, Glycylglycine,Monohydrochloride, Glycylglycine,N Glycylglycine
D000409	Alanine	A non-essential amino acid that occurs in high levels in its free state in plasma. It is produced from pyruvate by transamination. It is involved in sugar and acid metabolism, increases IMMUNITY, and provides energy for muscle tissue, BRAIN, and the CENTRAL NERVOUS SYSTEM.	Abufène,Alanine, L-Isomer,L-Alanine,Alanine, L Isomer,L Alanine,L-Isomer Alanine
D001692	Biological Transport	The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.	Transport, Biological,Biologic Transport,Transport, Biologic
D013237	Stereoisomerism	The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)	Molecular Stereochemistry,Stereoisomers,Stereochemistry, Molecular,Stereoisomer