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Spleen cells from hyperimmunized mice infected with Toxoplasma gondii were cultured in vitro with Toxoplasma specific antigen. The supernatant produced from the cells were termed lymphokines (LKs). The LKs were divided into 4 major fractions, namely: LKs-I, LKs-II, LKs-III and LKs-IV, according to the elution pattern on Sephadex G-100 gel columns. Partially purified LKs contained 2 MIF peaks, namely: MIF-I in LKs-II fraction and MIF-II in LKs-IV fraction. In this study, Toxoplasma growth inhibitory factor (Toxo-GIF), which inhibits the multiplication of Toxoplasma within non-immune macrophages in vitro, was separated by the same method as MIF separation, i.e. Sephadex G-100 gel filtration. Toxo-GIF activity was present in the LKs-II fraction in which MIF-I was also detected with a calculated molecular weight of 30,000 to 40,000. This murine LKs inhibited Toxoplasma multiplication only in murine macrophages but not in guinea pig macrophages or canine monocytes. Cytotoxic substances against macrophages were observed in the LKs-IV fraction, however, no Toxo-GIF was present in this fraction which in addition contained MIF-II with a calculated molecular weight of 3,000 to 5,000.
H Nagasawa, and
I Igarashi, and
T Matsumoto, and
H Sakurai, and
C Marbella, and
N Suzuki
September 1981,
Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...,
H Nagasawa, and
I Igarashi, and
T Matsumoto, and
H Sakurai, and
C Marbella, and
N Suzuki
November 1976,
Journal of immunology (Baltimore, Md. : 1950),
H Nagasawa, and
I Igarashi, and
T Matsumoto, and
H Sakurai, and
C Marbella, and
N Suzuki
July 1979,
Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie,
H Nagasawa, and
I Igarashi, and
T Matsumoto, and
H Sakurai, and
C Marbella, and
N Suzuki
September 2000,
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research,
H Nagasawa, and
I Igarashi, and
T Matsumoto, and
H Sakurai, and
C Marbella, and
N Suzuki
May 2013,
The Journal of biological chemistry,
H Nagasawa, and
I Igarashi, and
T Matsumoto, and
H Sakurai, and
C Marbella, and
N Suzuki
January 2002,
Critical care medicine,
H Nagasawa, and
I Igarashi, and
T Matsumoto, and
H Sakurai, and
C Marbella, and
N Suzuki
April 2003,
Expert opinion on therapeutic targets,
H Nagasawa, and
I Igarashi, and
T Matsumoto, and
H Sakurai, and
C Marbella, and
N Suzuki
January 2002,
Critical care medicine,
H Nagasawa, and
I Igarashi, and
T Matsumoto, and
H Sakurai, and
C Marbella, and
N Suzuki
December 2005,
Critical care medicine,
H Nagasawa, and
I Igarashi, and
T Matsumoto, and
H Sakurai, and
C Marbella, and
N Suzuki
August 1981,
Journal of immunology (Baltimore, Md. : 1950),
