[Ricin, the toxic protein of the castor-oil plant (Ricinus communis L). Structure and properties (author's transl)]. 1980

A A Lugnier, and E E Creppy, and G Dirheimer

Ricin is an extremely toxic phytotoxin from the castor-oil plant seeds. This toxin is different from the two phyto-hemagglutinins or lectins which are also present in the seeds and can be purified in two chromatographic steps. Studies on the physical and chemical properties of pure ricin are given (molecular weight: 65 750 daltons, glycoproteic nature, oses composition: 15 moles of mannose and 8 moles of N-acetyl-glucosamine per mole of ricin, aminoacids composition: 545, bicatenary structure: the toxin is formed by two polypeptide A and B chains linked together by a disulfure bond). Though ricin is resistant to proteolytic enzymes under normal conditions, we have found conditions in which tryptic hydrolysis of the toxin gives several peptides which retain toxicity. Two of them were purified. The LD50 on mice of ricin and its isolated toxic peptides were determined, the symptoms of ricin's intoxication were established on animals which died from a dramatic hepatonephritic injury, but always after a lag. Ricin has a cytostatic, and then a cytotoxic effect on cells in culture which are highly damaged (important membranous protrusions). The mechanism of ricin's action was studied. It inhibits elongation in protein synthesis in vivo as well as in vitro, by acting on ribosomes whether cytoplasmic, mitochondrial or chloroplastic. To act, the ricin A-chain must be activated by ribosomes which split the ricin molecule into its polypeptide chains; these ribosomes are then frozen by the toxin and became inefficients in protein synthesis.

UI MeSH Term Description Entries
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D002621 Chemistry A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012276 Ricin A protein phytotoxin from the seeds of Ricinus communis, the castor oil plant. It agglutinates cells, is proteolytic, and causes lethal inflammation and hemorrhage if taken internally. Castor Bean Lectin,Lectin, Castor Bean,Lectin, Ricinus,Ricin Toxin,RCA 60,RCA60,Ricin A Chain,Ricin B Chain,Ricin D,Ricin I,Ricinus Toxin,A Chain, Ricin,B Chain, Ricin,Ricinus Lectin,Toxin, Ricin,Toxin, Ricinus
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D055598 Chemical Phenomena The composition, structure, conformation, and properties of atoms and molecules, and their reaction and interaction processes. Chemical Concepts,Chemical Processes,Physical Chemistry Concepts,Physical Chemistry Processes,Physicochemical Concepts,Physicochemical Phenomena,Physicochemical Processes,Chemical Phenomenon,Chemical Process,Physical Chemistry Phenomena,Physical Chemistry Process,Physicochemical Phenomenon,Physicochemical Process,Chemical Concept,Chemistry Process, Physical,Chemistry Processes, Physical,Concept, Chemical,Concept, Physical Chemistry,Concept, Physicochemical,Concepts, Chemical,Concepts, Physical Chemistry,Concepts, Physicochemical,Phenomena, Chemical,Phenomena, Physical Chemistry,Phenomena, Physicochemical,Phenomenon, Chemical,Phenomenon, Physicochemical,Physical Chemistry Concept,Physicochemical Concept,Process, Chemical,Process, Physical Chemistry,Process, Physicochemical,Processes, Chemical,Processes, Physical Chemistry,Processes, Physicochemical

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