Biomaterial Database

[Rapid method for determining protein concentrations in the cells of microorganisms]. 1980

I A Gorina, and V I Iakovleva

The method of Lowry as modified by Peterson (who had changed concentrations of reagents and added a new reagent, sodium dodecyl sulfate) has been tested. It has been shown that the modified method can be applied for rapid measurement of protein in the suspension of bacterial cells. The effect of the following compounds on the protein estimation by the modified method in bovine serum albumin has been studied: potassium chloride, potassium phosphate, sucrose, EDTA, tris, ammonium acetate, Triton X-100, tyrosine, phenol, glycerol, 2-mercaptoethanol.

UI	MeSH Term	Description	Entries
D008722	Methods	A series of steps taken in order to conduct research.	Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D002954	Citrobacter	A genus of gram-negative, rod-shaped enterobacteria that can use citrate as the sole source of carbon.
D004926	Escherichia coli	A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.	Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D001426	Bacterial Proteins	Proteins found in any species of bacterium.	Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial